A PCR-based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological specimens.

Lineage 2 West Nile virus (WNV) strain has been involved in a severe outbreak of encephalitis in humans and equines which are in Europe. WNV molecular characterization is important for the development of diagnostic tests, and to obtain molecular information, which is necessary for epidemiological investigations in areas at risk of virus transmission. For whole-genome sequencing of strains of WNV lineage 2, directly from biological specimens, PCR-based NGS protocol developed.

This method is applied to the WNV-positive specimens were obtained from animals, human and mosquito hosts in Greece. Outcomes of the application shows that, even in the case of a low viral titers, developed PCR-based NGS approach capable of providing whole genome sequence of strain lineage 2 WNV.

This study evaluated the performance of biological denitrification in anoxic biofilm sequencing batch reactor (ASBBR) and nitrous oxide (N2O) emissions. After 90 days of operation, chemical oxygen demand of waste and the amount of nitrogen elimination of high-efficiency 94.8% and 95.0%, respectively.

Both polysaccharides and the protein content is reduced in the bound EPS (TB-EPS) and loosely bound EPS (LB-EPS) after the formation of biofilms. According to a typical cycle, the rate of release of N2O associated with the concentration of free nitric acid (FNA) with a maximum value of 3.88 mg / min and the total conversion rate of 1.27%. Two components identified from the model EEM-PARAFAC in soluble microbial products (SMP).

Proteins such as substance for one component changed significantly in the process of denitrification, while humic and fulvic acid-like-like substance for the second component remained relatively stable. Results of high throughput sequencing showed that Lysobacter, Tolumonas and Thauera is the dominant genera, showed the co-existence of autotrophic and heterotrophic denitrifiers in ASBBR.

TSH2 Antibody
45554-50ul 50ul
EUR 224.4

TSH2 Antibody
DF8874 200ul
EUR 420

TSH2 Antibody
DF8874-100ul 100ul
EUR 168
Description: WB,ELISA(peptide)

TSH2 Antibody
DF8874-200ul 200ul
EUR 210
Description: WB,ELISA(peptide)

TSH2 Antibody
E11-11748C 100μg
EUR 225
Description: Available in various conjugation types.

TSH2 Antibody
E19-8874-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

TSH2 Antibody
E19-8874-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

TSH2 Antibody
MBS8517885-005mg 0.05mg
EUR 235

TSH2 Antibody
MBS8517885-01mg 0.1mg
EUR 305

TSH2 Antibody
MBS8517885-01mLAF405M 0.1mL(AF405M)
EUR 465

TSH2 Antibody
MBS8517885-01mLAF546 0.1mL(AF546)
EUR 465

TSH2 Antibody
MBS8517885-01mLAF750 0.1mL(AF750)
EUR 465

TSH2 Antibody
MBS859669-01mg 0.1mg
EUR 305

TSH2 Antibody
MBS859669-01mLAF405L 0.1mL(AF405L)
EUR 465

TSH2 Antibody
MBS859669-01mLAF405S 0.1mL(AF405S)
EUR 465

TSH2 Antibody
MBS859669-01mLAF610 0.1mL(AF610)
EUR 465

TSH2 Antibody
MBS859669-01mLAF635 0.1mL(AF635)
EUR 465

TSH2 Antibody
MBS9505487-005mL 0.05mL
EUR 285

TSH2 Antibody
MBS9505487-01mL 0.1mL
EUR 385

TSH2 Antibody
MBS9505487-5x01mL 5x0.1mL
EUR 1590

TSH2 Antibody
MBS9609191-01mL 0.1mL
EUR 260

TSH2 Antibody
MBS9609191-02mL 0.2mL
EUR 305

TSH2 Antibody
MBS9609191-5x02mL 5x0.2mL
EUR 1220

TSH2 Blocking Peptide
DF8874-BP 1mg
EUR 234

TSH2 Blocking Peptide
MBS9627895-1mg 1mg
EUR 380

TSH2 Blocking Peptide
MBS9627895-5x1mg 5x1mg
EUR 1650

TSH2 Conjugated Antibody
C45554 100ul
EUR 476.4

TSH2 Conjugated Antibody
MBS9453506-01mLAF350 0.1mL(AF350)
EUR 480

TSH2 Conjugated Antibody
MBS9453506-01mLAF405 0.1mL(AF405)
EUR 480

TSH2 Conjugated Antibody
MBS9453506-01mLAF488 0.1mL(AF488)
EUR 480

TSH2 Conjugated Antibody
MBS9453506-01mLAF555 0.1mL(AF555)
EUR 480

TSH2 Conjugated Antibody
MBS9453506-01mLBiotin 0.1mL(Biotin)
EUR 480

TSH2 Antibody (N-term)
MBS9202384-008mL 0.08mL
EUR 210

TSH2 Antibody (N-term)
MBS9202384-04mL 0.4mL
EUR 430

TSH2 Antibody (N-term)
MBS9202384-5x04mL 5x0.4mL
EUR 1910

Teashirt Homolog 2 (TSH2) Antibody
abx032471-400l 400 µl
EUR 518.75

Teashirt Homolog 2 (TSH2) Antibody
abx032471-400ul 400 ul
EUR 627.6

Teashirt Homolog 2 (TSH2) Antibody
abx032471-80l 80 µl
EUR 250

Teashirt Homolog 2 (TSH2) Antibody
20-abx219422
  • Ask for price
  • Ask for price
  • 50 ug
  • 100 ug

Anti-TSH2 TSHZ2 Antibody
A08863 100ul
EUR 335
Description: Boster Bio Anti-TSH2 TSHZ2 Antibody (Catalog# A08863). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.

TSH2 Antibody (N-term) Blocking peptide
MBS9225603-INQUIRE INQUIRE Ask for price

Rabbit polyclonal TSH2 Antibody (N-term)
TA324804 400 µl Ask for price

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218)
MBS646528-02mL 0.2mL
EUR 695

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218)
MBS646528-5x02mL 5x0.2mL
EUR 2975

OAAB02705-400UL - TSH2 Antibody - N-terminal region
OAAB02705-400UL 400ul
EUR 389

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (AP)
MBS6359485-02mL 0.2mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (AP)
MBS6359485-5x02mL 5x0.2mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (APC)
MBS6359486-02mL 0.2mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (APC)
MBS6359486-5x02mL 5x0.2mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (FITC)
MBS6359488-02mL 0.2mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (FITC)
MBS6359488-5x02mL 5x0.2mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (PE)
MBS6359495-02mL 0.2mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (PE)
MBS6359495-5x02mL 5x0.2mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (Biotin)
MBS6359487-02mL 0.2mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (Biotin)
MBS6359487-5x02mL 5x0.2mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 405)
MBS6359490-01mL 0.1mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 405)
MBS6359490-5x01mL 5x0.1mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 490)
MBS6359491-01mL 0.1mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 490)
MBS6359491-5x01mL 5x0.1mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 550)
MBS6359492-01mL 0.1mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 550)
MBS6359492-5x01mL 5x0.1mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 650)
MBS6359493-01mL 0.1mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 650)
MBS6359493-5x01mL 5x0.1mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 750)
MBS6359494-01mL 0.1mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 750)
MBS6359494-5x01mL 5x0.1mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (Azide free) (HRP)
MBS6359489-02mL 0.2mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (Azide free) (HRP)
MBS6359489-5x02mL 5x0.2mL
EUR 4250

Rabbit polyclonal anti-TSH2 antibody
TA316092 100 µl Ask for price

TSH beta (TSHB) mouse monoclonal antibody, clone TSH220, Purified
AM10041PU-N 1 ml Ask for price

TSH beta (TSHB) mouse monoclonal antibody, clone TSH220, Purified
AM10041PU-S 500 µl Ask for price

TSH beta (TSHB) mouse monoclonal antibody, clone TSH-116, Aff - Purified
AM03069PU-N 100 µg Ask for price

TSH beta (TSHB) mouse monoclonal antibody, clone TSH-2, HRP
AM09312HR-N 200 µl Ask for price

TSH beta (TSHB) mouse monoclonal antibody, clone TSH-2, Aff - Purified
AM09312PU-N 500 µg Ask for price

TSH1 Antibody
ABD8843 100 ug
EUR 525.6

TSH3 Antibody
ABD10052 100 ug
EUR 525.6

TSH1 Antibody
ABD13297 100 ug
EUR 525.6

TSH1 Antibody
8C11240 50ug
EUR 368
Description: TSH1 Antibody

TSH1 antibody
70R-31967 100 ug
EUR 294
Description: Rabbit polyclonal TSH1 antibody

TSH3 Antibody
44481-100ul 100ul
EUR 302.4

TSH3 Antibody
44481-50ul 50ul
EUR 224.4

TSH1 Antibody
45535-100ul 100ul
EUR 302.4

TSH1 Antibody
45535-50ul 50ul
EUR 224.4

TSH3 Antibody
DF10052 200ul
EUR 420

TSH3 Antibody
DF10052-100ul 100ul
EUR 168
Description: WB,ELISA(peptide)

TSH3 Antibody
DF10052-200ul 200ul
EUR 210
Description: WB,ELISA(peptide)

TSH1 Antibody
DF8843 200ul
EUR 420

TSH1 Antibody
DF8843-100ul 100ul
EUR 168
Description: WB,IHC,ELISA(peptide)

TSH1 Antibody
DF8843-200ul 200ul
EUR 210
Description: WB,IHC,ELISA(peptide)

TSH3 Antibody
C46888-100ul 100μl
EUR 217
Description: TSH3 Rabbit Polyclonal Antibody

TSH3 Antibody
C46888-50ul 50μl
EUR 143.5
Description: TSH3 Rabbit Polyclonal Antibody

TSH1 Antibody
E11-11240C 100μg
EUR 225
Description: Available in various conjugation types.

TSH3 Antibody
E19-10052-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

TSH3 Antibody
E19-10052-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

TSH1 Antibody
E19-8843-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

TSH1 Antibody
E19-8843-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

TSH1 Antibody
MBS8517854-005mg 0.05mg
EUR 235

TSH1 Antibody
MBS8517854-01mg 0.1mg
EUR 305

TSH1 Antibody
MBS8517854-01mLAF405M 0.1mL(AF405M)
EUR 465

TSH1 Antibody
MBS8517854-01mLAF546 0.1mL(AF546)
EUR 465

TSH1 Antibody
MBS8517854-01mLAF750 0.1mL(AF750)
EUR 465

TSH3 Antibody
MBS8513330-005mg 0.05mg
EUR 235

TSH3 Antibody
MBS8513330-01mg 0.1mg
EUR 305

TSH3 Antibody
MBS8513330-01mLAF405M 0.1mL(AF405M)
EUR 465

TSH3 Antibody
MBS8513330-01mLAF546 0.1mL(AF546)
EUR 465

TSH3 Antibody
MBS8513330-01mLAF750 0.1mL(AF750)
EUR 465

TSH1 Antibody
MBS859525-01mg 0.1mg
EUR 305

TSH1 Antibody
MBS859525-01mLAF405L 0.1mL(AF405L)
EUR 465

TSH1 Antibody
MBS859525-01mLAF405S 0.1mL(AF405S)
EUR 465

TSH1 Antibody
MBS859525-01mLAF610 0.1mL(AF610)
EUR 465

TSH1 Antibody
MBS859525-01mLAF635 0.1mL(AF635)
EUR 465

TSH3 Antibody
MBS9602911-01mL 0.1mL
EUR 260

TSH3 Antibody
MBS9602911-02mL 0.2mL
EUR 305

TSH3 Antibody
MBS9602911-5x02mL 5x0.2mL
EUR 1220

TSH1 Antibody
MBS9505305-005mL 0.05mL
EUR 285

TSH1 Antibody
MBS9505305-01mL 0.1mL
EUR 385

TSH1 Antibody
MBS9505305-5x01mL 5x0.1mL
EUR 1590

TSH1 Antibody
MBS9609160-01mL 0.1mL
EUR 260

TSH1 Antibody
MBS9609160-02mL 0.2mL
EUR 305

TSH1 Antibody
MBS9609160-5x02mL 5x0.2mL
EUR 1220

Thyroid Stimulating Hormone (TSH, Thyrotropin beta, TSH-beta, TSH-B, TSHB) (APC)
MBS6269201-01mL 0.1mL
EUR 1275

Thyroid Stimulating Hormone (TSH, Thyrotropin beta, TSH-beta, TSH-B, TSHB) (APC)
MBS6269201-5x01mL 5x0.1mL
EUR 5585

Thyroid Stimulating Hormone (TSH, Thyrotropin beta, TSH-beta, TSH-B, TSHB) (PE)
MBS6252219-01mL 0.1(mL
EUR 1275

Thyroid Stimulating Hormone (TSH, Thyrotropin beta, TSH-beta, TSH-B, TSHB) (PE)
MBS6252219-5x01mL 5x0.1mL
EUR 5585

Thyroid Stimulating Hormone (TSH, Thyrotropin beta, TSH-beta, TSH-B, TSHB) (HRP)
MBS6251283-01mL 0.1(mL
EUR 1275

Thyroid Stimulating Hormone (TSH, Thyrotropin beta, TSH-beta, TSH-B, TSHB) (HRP)
MBS6251283-5x01mL 5x0.1mL
EUR 5585

Thyroid Stimulating Hormone (TSH, Thyrotropin beta, TSH-beta, TSH-B, TSHB) (AP)
MBS6248349-01mL 0.1(mL
EUR 1275

Thyroid Stimulating Hormone (TSH, Thyrotropin beta, TSH-beta, TSH-B, TSHB) (AP)
MBS6248349-5x01mL 5x0.1mL
EUR 5585

TSH3 Blocking Peptide
DF10052-BP 1mg
EUR 234

TSH1 Blocking Peptide
DF8843-BP 1mg
EUR 234

TSH1 Blocking Peptide
MBS9627865-1mg 1mg
EUR 380

TSH1 Blocking Peptide
MBS9627865-5x1mg 5x1mg
EUR 1650

TSH3 Blocking Peptide
MBS9620388-1mg 1mg
EUR 380

TSH3 Blocking Peptide
MBS9620388-5x1mg 5x1mg
EUR 1650

Thyroid Stimulating Hormone (TSH, Thyrotropin beta, TSH-beta, TSH-B, TSHB) (FITC)
MBS6250256-01mL 0.1(mL
EUR 1275

Thyroid Stimulating Hormone (TSH, Thyrotropin beta, TSH-beta, TSH-B, TSHB) (FITC)
MBS6250256-5x01mL 5x0.1mL
EUR 5585

TSH/ Rat TSH ELISA Kit
ELA-E0463r 96 Tests
EUR 1063.2

Thyroid Stimulating Hormone (TSH, Thyrotropin beta, TSH-beta, TSH-B, TSHB) (Biotin)
MBS6249356-01mL 0.1(mL
EUR 1275

Thyroid Stimulating Hormone (TSH, Thyrotropin beta, TSH-beta, TSH-B, TSHB) (Biotin)
MBS6249356-5x01mL 5x0.1mL
EUR 5585

TSH3 Polyclonal Antibody
ABP60781-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of TSH3 from Human. This TSH3 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human TSH3 protein