Deconvolution of autoencoders to learn biological regulatory modules from single cell mRNA sequencing data.

Deconvolution of autoencoders to learn biological regulatory modules from single cell mRNA sequencing data.

by

methods of unsupervised machine learning (learning to) has demonstrated its usefulness in a single cell mRNA noisy data-sequencing (scRNA-seq), where models generalize well, although a zero-inflation of the data. A class of neural networks, namely autoencoders, has been useful for denoising a single cell data, imputed values ​​and dimensions reduction.Here missing, we present a striking feature with the potential to greatly improve the usability of autoencoders:

With special training, autoencoder not only able to generalize on data, but also to tease apart biologically meaningful modules, which we found encoded in the representation of the network layer. Our models can, from the data scRNA-seq, Delineate biologically meaningful modules that regulate dataset, as well as the information given to the active module in every single cell. Importantly, most of these modules can be explained by the known biological function, such as that provided by Hallmark genes sets.We found that training was adjusted from autoencoder allows for deconvolute biology module embedded in the data, without assumptions. By comparison with the gene signatures of canonical pathways we see that the modules are directly interpreted.

The scope of the present invention has important implications, as it allows to elaborate on specific effects of cell behind the driver. Compared to the other dimension reduction method, or model to be followed for classification, our approach has benefits both deal with both the nature of the zero-increase of scRNA-seq, and validate that the model captures the relevant information, to establish the relationship between the input and translate data. In perspective, our model in combination with classifying this method is able to provide information about a given single cell subtypes belong to, as well as the biological functions that determine membership.

Deconvolution of autoencoders to learn biological regulatory modules from single cell mRNA sequencing data.
Deconvolution of autoencoders to learn biological regulatory modules from single cell mRNA sequencing data.

This study evaluated nitrogen removal methods Novel shortcut using a mixture consortium of microalgae, enriched ammonia oxidizing bacteria (AOB) and methanol utilizing denitrifier (MUD) in a photo-sequencing batch reactor (PSBR) for treating ammonium-rich wastewater (ARWW).

Alternating periods of light and darkness followed to obtain complete elimination of biological nitrogen (BNR) without external aeration and with the addition of methanol as a sole carbon source, respectively. The results showed that the effect of NH4 + is oxidized to NO 2 by AOB during the light period at a rate of 8:09 mg NH4 + -N L-1h-1

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EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (APC)
MBS6359486-02mL 0.2mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (APC)
MBS6359486-5x02mL 5x0.2mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (FITC)
MBS6359488-02mL 0.2mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (FITC)
MBS6359488-5x02mL 5x0.2mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (PE)
MBS6359495-02mL 0.2mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (PE)
MBS6359495-5x02mL 5x0.2mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218)
MBS646528-02mL 0.2mL
EUR 695

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218)
MBS646528-5x02mL 5x0.2mL
EUR 2975

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (Biotin)
MBS6359487-02mL 0.2mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (Biotin)
MBS6359487-5x02mL 5x0.2mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (Azide free) (HRP)
MBS6359489-02mL 0.2mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (Azide free) (HRP)
MBS6359489-5x02mL 5x0.2mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 405)
MBS6359490-01mL 0.1mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 405)
MBS6359490-5x01mL 5x0.1mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 490)
MBS6359491-01mL 0.1mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 490)
MBS6359491-5x01mL 5x0.1mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 550)
MBS6359492-01mL 0.1mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 550)
MBS6359492-5x01mL 5x0.1mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 650)
MBS6359493-01mL 0.1mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 650)
MBS6359493-5x01mL 5x0.1mL
EUR 4250

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 750)
MBS6359494-01mL 0.1mL
EUR 980

TSH2, NT (TSHZ2, C20orf17, TSH2, ZNF218, Teashirt homolog 2, Ovarian cancer-related protein 10-2, Zinc finger protein 218) (MaxLight 750)
MBS6359494-5x01mL 5x0.1mL
EUR 4250

TSH2 Antibody
8C11748 50ug
EUR 368
Description: TSH2 Antibody

TSH2 Antibody
45554-100ul 100ul
EUR 302.4

TSH2 Antibody
45554-50ul 50ul
EUR 224.4

TSH2 antibody
70R-32153 100 ug
EUR 294
Description: Rabbit polyclonal TSH2 antibody

TSH3 Antibody
E19-10052-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

TSH3 Antibody
E19-10052-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

TSH2 Antibody
E11-11748C 100μg
EUR 225
Description: Available in various conjugation types.

TSH2 Antibody
E19-8874-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

TSH2 Antibody
E19-8874-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

TSH3 Antibody
44481-100ul 100ul
EUR 302.4

TSH3 Antibody
44481-50ul 50ul
EUR 224.4

TSH2 Antibody
ABD8874 100 ug
EUR 525.6

TSH3 Antibody
ABD10052 100 ug
EUR 525.6

TSH3 Antibody
C46888-100ul 100μl
EUR 217
Description: TSH3 Rabbit Polyclonal Antibody

TSH3 Antibody
C46888-50ul 50μl
EUR 143.5
Description: TSH3 Rabbit Polyclonal Antibody

TSH2 Antibody
DF8874 200ul
EUR 420

TSH2 Antibody
DF8874-100ul 100ul
EUR 168
Description: WB,ELISA(peptide)

TSH2 Antibody
DF8874-200ul 200ul
EUR 210
Description: WB,ELISA(peptide)

TSH3 Antibody
DF10052 200ul
EUR 420

TSH3 Antibody
DF10052-100ul 100ul
EUR 168
Description: WB,ELISA(peptide)

TSH3 Antibody
DF10052-200ul 200ul
EUR 210
Description: WB,ELISA(peptide)

TSH2 Antibody
MBS8517885-005mg 0.05mg
EUR 235

TSH2 Antibody
MBS8517885-01mg 0.1mg
EUR 305

TSH2 Antibody
MBS8517885-01mLAF405M 0.1mL(AF405M)
EUR 465

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