Do you cov me? Effect of coverage reduction on species identification and genome reconstruction in complex biological matrices by metagenome shotgun high-throughput sequencing.

At the end of the period at 20.0 mg HS - S / L, specific levels of consumption and the formation of ammonium nitrate is 15 and 55% lower than the values ​​in each control period without the addition of sulphide, which indicates that the mud had better adaptation metabolic activities of the oxidizer ammonium nitrite oxidation activity. sludge obtained sulfide oxidation capacity higher throughout the cycle. Bacterial population dynamics assessment showed that ammonium oxidizing bacteria (AOB) community more diverse and stable than nitrite-oxidizing bacteria (NOB) community. Use of a consortium with previous nitrification activity stabilized in the SBR may be an alternative to eliminate simultaneous nitrification and ammonium sulfide by oxidation of sulphides and applied for wastewater treatment with ammonium and sulfide.

Metagenomics Shotgun sequencing is a powerful tool for the characterization of complex biological matrices, analysis of prokaryotic and eukaryotic organisms allowed in a single experiment, with the possibility of de novo reconstruction of the entire metagenome or a set of genes of interest. One of the main factors that limit the use of metagenomics rifle … Read more