Targeted sequencing analysis of cell-free DNA from metastatic non-small-cell lung cancer patients: clinical and biological implications.

Sequencing artifacts, clonal hematopoietic potential mutation indeterminate (CHIP) and the heterogeneity of the tumor has been hypothesized to contribute to the low suitability of tissue and cell-free DNA (cfDNA) with a targeted molecular profiling sequencing.We cfDNA analyzed by targeted sequencing of Hypersaline wastewater may pose a threat to the biological wastewater treatment process. samples from 30 and 77 EGFR-mutant wild-type EGFR lung cancer metastatic non-small cell (mNSCLC) patients. discordant cases solved with digital droplet PCR (ddPCR) .

With cfDNA testing from a healthy donor, we developed an algorithm to recognize the sequencing artifacts. Applying this method to cfDNA of patients mNSCLC, EGFR mutations detected with good sensitivity (76.7%) and specificity (97.4%). In contrast, the sensitivity and specificity for the KRAS variant was 61.5% and 93.8%, respectively. All of EGFR and KRAS variant was detected in the plasma but not in the network is confirmed by ddPCR, thus excluding sequencing artifacts. In a small proportion of cases, KRAS mutations are found in plasma samples was confirmed in tumor tissue showed tumor heterogeneity.

KRAS variant was found to be possible sub-clonal compared with the EGFR mutation, and the correlation between the clonal origin and frequency of detection in plasma was found. In the case of both EGFR and KRAS variant in cfDNA, we can show the presence of KRAS in the tumor tissue variants associated with lack of response to tyrosine kinase inhibitors (TKI) .Although sequencing artifacts can be identified in order cfDNA targets, tumor heterogeneity and CHIP are likely to affect the suitability between plasma and tissue testing.

Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient.

Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient. Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.
Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient. Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.

Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.

Trypan Blue Assay Kit

AR1175 1kit (for 1000 assays)
EUR 78

Trypan Blue (C.I. 23850)

GT3970-100G 100 g
EUR 132

Trypan Blue (C.I. 23850)

GT3970-25G 25 g
EUR 74.4

100mL Trypan Blue Solution wv

25-900-CI EACH
EUR 23.44

OPTIONAL BLUE FILTER (497NM)

GDBL-BLUE 1/pk
EUR 1298.4
Description: Lab Equipment; Axygen Branded EQ

conversion screen uv/blue

VLFC26-BLUE ea
EUR 727.2

Trypan Blue, sodium salt *CAS 72-57-1*

2450 100 g
EUR 262.8

Trypan Blue, sodium salt *10 mM aqueous solution*

2453 10 mL
EUR 262.8

Cell Counting Kit (CCK)

20-abx098245
  • EUR 627.60
  • EUR 493.20
  • EUR 861.60
  • EUR 560.40
  • 10 ml
  • 1 ml
  • 30 ml
  • 5 ml

Cell Counting Kit-8

HY-K0301 120 mL (12000 T)
EUR 1362

Trypan red

HY-D0983 5mg
EUR 639.6

CCK-8 Cell Counting Kit

A311-01 500 rxn
EUR 214.8

CCK-8 Cell Counting Kit

A311-02 1000 rxn
EUR 280.8

Counting Slide 2 Cell Mcmaster

HAE2030 EACH
EUR 173.28

Cell Counting Pot Square Base

CEL10500 PK200
EUR 53.58

Organoid Counting Software

6749-OC EACH
EUR 6542.46

Trypan Blue, sodium salt *UltraPure grade* *Purified to eliminate fluorescent impurities*

2452 10 g
EUR 262.8

Cell Counting Kit-8 (CCK-8)

B34302 5 mL (500 ractions)
EUR 156
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

Cell Counting Kit-8 (CCK-8)

B34304 25 mL (2500 ractions)
EUR 378
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

Cell Counting Kit-8 (CCK-8)

AR1160 5 mL (for 500 assays, 10μL per well)
EUR 157.2

Cell Counting Kit-8 (CCK-8)

K1018-1 1x1 ml (100 tests)
EUR 87.6

Cell Counting Kit-8 (CCK-8)

K1018-10 2x5 ml (1000 tests)
EUR 199.2

Cell Counting Kit-8 (CCK-8)

K1018-100 20x5 ml (10000 tests)
EUR 1062

Cell Counting Kit-8 (CCK-8)

K1018-30 6x5 ml (3000 tests)
EUR 393.6

Cell Counting Kit-8 (CCK-8)

K1018-5 1x5 ml (500 tests)
EUR 142.8

Plastic cell counting slides Kovas Slide

DD50126 EACH
EUR 253.08

Counting Chamber Helber Bacteria

COU9100 EACH
EUR 111.72

Coverslip for counting chamber

Z5000 PK10
EUR 19.38

CORNING COUNTING CHAMBER FOR CORNING CELL COUNTER

480200 1/pk
EUR 170.4
Description: Lab Equipment; Cell Counters

Cell Counting Kit-8 (CCK8) Cell Viability Assay Kit

abx090677-500tests 500 tests
EUR 472.8

Bacterial Counting Colorimetric Assay Kit

K511-2500
EUR 744

Bacterial Counting Colorimetric Assay Kit

K511-500
EUR 418.8

Fast Read Disposable Counting Chambers

390497 PK100
EUR 159.6

BRAND(TM) BLAUBRAND(R) COUNTING CHAMBER

BR717820-1EA EACH
EUR 81.51

Trypan Purple™ *0.1 M aqueous solution*

2465 10 mL
EUR 262.8

Trypan Purple™ *0.1 M aqueous solution*

2466 100 mL
EUR 1051.2

Counting Chamber Improved Neubauer 2 x Bright

HAE2118 EACH
EUR 91.2

Synbiosis AutoCOL Fully Automated Colony Counting System

COU2020 EACH
EUR 119700

ECOS Blue (XL1-Blue

FYE107-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE107-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE108-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE108-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE109-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE109-80VL 100 µl x 10 vials Ask for price

Trypan Red Plus™ *0.1 M aqueous solution*

2456 10 mL
EUR 262.8

Trypan Red Plus™ *0.1 M aqueous solution*

2457 100 mL
EUR 837.6

Blue Chemically Competent Cell (TetR)

20-abx098068
  • EUR 510.00
  • EUR 543.60
  • 1 ml
  • 2 ml

Trypan UltraBlue™, sodium salt *0.1 M aqueous solution*

2455 1 mL
EUR 262.8

Bromophenol Blue

CH019 25 g
EUR 158.4

Bromophenol Blue

CH020 50 g
EUR 199.2

Bromophenol Blue

CH021 100 g
EUR 272.4

Evans Blue

20-abx082082
  • EUR 210.00
  • EUR 260.40
  • 1 g
  • 5 g

Bromphenol Blue

20-abx082184
  • EUR 276.00
  • EUR 226.80
  • 25 g
  • 5 g

Evans Blue

20-abx082237
  • EUR 226.80
  • EUR 276.00
  • 1 g
  • 5 g

Bromphenol Blue

20-abx082376
  • EUR 292.80
  • EUR 226.80
  • 25 g
  • 5 g

Toluidine Blue

abx082425-5g 5 g
EUR 226.8

Bromophenol Blue

abx082607-10g 10 g
EUR 243.6

Phthalocyanine blue

20-abx181340
  • EUR 393.60
  • EUR 260.40
  • EUR 895.20
  • 100 g
  • 25 g
  • 500 g

Bromophenol blue

abx183916-500ml 500 ml
EUR 276

Bromophenol blue

abx183916-5l 5l
EUR 710.4

Evans Blue

abx186080-125g 125g
EUR 543.6

Bromophenol blue

BB2230 25g
EUR 82.97

Bromophenol Blue

B7774-5000 5 g
EUR 104.4
Description: IC50: N/ABromophenol blue (3',3",5',5"-tetrabromophenolsulfonphthalein) is widely used in gel loading buffers. Bromophenol blue is experimentally used as acolor marker, apH indicator, and a dye.

Bromophenol Blue

G114 5.0 g
EUR 122.4

Evans Blue

HY-B1102 100mg
EUR 142.8

Bromothymol Blue

HY-D0012 1g
EUR 142.8

Diphenyl Blue

HY-D0970 10mM/1mL
EUR 135.6

Calcein Blue

HY-101887 25mg
EUR 142.8

Methylene Blue

HY-14536 100mg
EUR 142.8

Thiazolyl Blue

HY-15924 10mM/1mL
EUR 135.6

Meldola's blue

GT0259-10G 10 g
EUR 103.2

Meldola's blue

GT0259-25G 25 g
EUR 160.8

Meldola's blue

GT0259-50G 50 g
EUR 255.6

Bromochlorophenol Blue

GT2674-100MG 100 mg
EUR 93.6

Bromochlorophenol Blue

GT2674-1G 1 g
EUR 208.8

Bromophenol Blue

GT2874-100G 100 g
EUR 151.2

Bromophenol Blue

GT2874-10G 10 g
EUR 62.4

Bromophenol Blue

GT2874-25G 25 g
EUR 79.2

Bromophenol Blue

GT2874-50G 50 g
EUR 103.2

Bromophenol Blue

GT2874-5G 5 g
EUR 54

Hydroxynaphthol blue

GT3182-100G 100 g
EUR 284.4

Hydroxynaphthol blue

GT3182-10G 10 g
EUR 93.6

Hydroxynaphthol blue

GT3182-25G 25 g
EUR 132

Hydroxynaphthol blue

GT3182-50G 50 g
EUR 199.2

Bromothymol Blue

GT6837-10G 10 g
EUR 56.4

Bromothymol Blue

GT6837-25G 25 g
EUR 73.2

Bromothymol Blue

GT6837-50G 50 g
EUR 103.2

Bromothymol Blue

GT6837-5G 5 g
EUR 50.4

Xylenol Blue

GT7988-1G 1 g
EUR 55.2

Xylenol Blue

GT7988-25G 25 g
EUR 180

Xylenol Blue

GT7988-5G 5 g
EUR 93.6

Bromoxylenol Blue

GT8506-10G 10 g
EUR 170.4

Bromoxylenol Blue

GT8506-1G 1 g
EUR 55.2

Bromoxylenol Blue

GT8506-5G 5 g
EUR 112.8

Thymol Blue

GT9755-100G 100 g
EUR 180

Thymol Blue

GT9755-10G 10 g
EUR 60

Thymol Blue

GT9755-25G 25 g
EUR 84

Thymol Blue

GT9755-50G 50 g
EUR 112.8

Thymol Blue

GT9755-5G 5 g
EUR 50.4

CellQuanti-Blue Cell Viability Assay Kits

CQBL-05K 5000
EUR 265.2
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 5000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

CellQuanti-Blue Cell Viability Assay Kits

CQBL-10K 10000
EUR 408
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 10000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

CellCount-Blue Cell Proliferation Assay Kit

C0100-025 250 Assays
EUR 133.2

CellCount-Blue Cell Proliferation Assay Kit

C0100-050 500 Assays
EUR 160.8

CellCount-Blue Cell Proliferation Assay Kit

C0100-100 1000 Assays
EUR 225.6

CellCount-Blue Cell Proliferation Assay Kit

C0100-500 5000 Assays
EUR 639.6

Blue Chemically Competent Cell (TetR, CamR)

20-abx098069
  • EUR 526.80
  • EUR 577.20
  • 1 ml
  • 2 ml

Container 125ml PP Blue+Label+Blue Cap

CON1013 PK380
EUR 208.64

Snap Cap 11mm Blue Si/ PTFE Blue

CHR3550 PK1000
EUR 1045.38

Snap Cap 11mm Blue Si/PTFE Blue

CHR3552 PK1000
EUR 436.62

Embedding Cassettes

M480-6-BLUE 1 ea.
EUR 232.8

Cassettes in QuickLoad Sleeves

M480-6SL-BLUE 1 ea.
EUR 340.8

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135 96 assays
EUR 985.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135-5 5 x 96 assays
EUR 4027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140 96 assays
EUR 1027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140-5 5 x 96 assays
EUR 4179.6
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

293AD Cell Line

AD-100 1 vial
EUR 553.2
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 609.6
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 609.6
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 609.6
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

TER-119 / Erythroid Cell Antibody (CF-Blue)

20-abx200924
  • EUR 543.60
  • EUR 360.00
  • 100 ug
  • 25 ug

Cell Strainer 40um Blue Sterile Ind. Wrapped

ARG1218 PK50
EUR 185.82

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325 96 assays
EUR 1027.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325-5 5 x 96 assays
EUR 4033.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

Container 40ml PP Blue+Label+Blue Cap Select

CON1025 PK1000
EUR 220.43

Cell Meter™ Cell Viability Assay Kit *Blue Fluorescence*

22785 500 Tests
EUR 262.8

Cell Explorer™ Live Cell Labeling Kit *Blue Fluorescence*

22606 200 Tests
EUR 262.8

Cell Explorer™ Live Cell Tracking Kit *Blue Fluorescence*

22620 200 Tests
EUR 262.8

DNASE & TOLUIDINE BLUE

D04-103-10kg 10 kg
EUR 11338.8

DNASE & TOLUIDINE BLUE

D04-103-2Kg 2 Kg
EUR 2511.6

DNASE & TOLUIDINE BLUE

D04-103-500g 500 g
EUR 726

Blue Gravity Column

BSP089 1Column, 1 UNIT
EUR 77.75

Brilliant Blue G

C5579-100000 100 g
EUR 162
Description: Coomassie Brilliant Blue G-250 is used for protein staining in SDS-PAGE, Blue Native PAGE, and the Bradford Method, with high band visibility.

Thiolite™ Blue

21507 5 mg
EUR 158.4

Amplite™ Blue

11005 25 mg
EUR 211.2

CytoTell™ Blue

22251 500 Tests
EUR 158.4

CytoTell™ Blue

22252 1000 Tests
EUR 211.2

LysoBrite™ Blue

22642 500 Tests
EUR 211.2

Evans Blue Tetrasodium

2586-1G
EUR 151.2

Dextran blue 2000

20-abx082076
  • EUR 260.40
  • EUR 427.20
  • 1 g
  • 5 g

Alcian Blue 8GX

abx082097-1g 1 g
EUR 260.4

Alcian Blue 8GX

abx082417-1g 1 g
EUR 243.6

Aniline Blue Solution

ABP999 1000 ml
EUR 159.6

Alkali blue 6B

AB0016 25g
EUR 80.88

Aniline Blue Solution

ABP125 125 ml
EUR 86.4

Aniline Blue Solution

ABP500 500 ml
EUR 127.2

C.I.Var Blue 6

abx180699-25g 25 g
EUR 661.2

C.I.Reactive Blue 19

20-abx181246
  • EUR 610.80
  • EUR 326.40
  • 25 g
  • 5 g

C.I.Solvent Blue 36

20-abx181247
  • EUR 510.00
  • EUR 343.20
  • EUR 243.60
  • 100 g
  • 25 g
  • 5 g

Pigment Blue 29

abx185539-100g 100 g
EUR 444

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