Targeted sequencing analysis of cell-free DNA from metastatic non-small-cell lung cancer patients: clinical and biological implications.

Sequencing artifacts, clonal hematopoietic potential mutation indeterminate (CHIP) and the heterogeneity of the tumor has been hypothesized to contribute to the low suitability of tissue and cell-free DNA (cfDNA) with a targeted molecular profiling sequencing.We cfDNA analyzed by targeted sequencing of Hypersaline wastewater may pose a threat to the biological wastewater treatment process. samples from 30 and 77 EGFR-mutant wild-type EGFR lung cancer metastatic non-small cell (mNSCLC) patients. discordant cases solved with digital droplet PCR (ddPCR) .

With cfDNA testing from a healthy donor, we developed an algorithm to recognize the sequencing artifacts. Applying this method to cfDNA of patients mNSCLC, EGFR mutations detected with good sensitivity (76.7%) and specificity (97.4%). In contrast, the sensitivity and specificity for the KRAS variant was 61.5% and 93.8%, respectively. All of EGFR and KRAS variant was detected in the plasma but not in the network is confirmed by ddPCR, thus excluding sequencing artifacts. In a small proportion of cases, KRAS mutations are found in plasma samples was confirmed in tumor tissue showed tumor heterogeneity.

KRAS variant was found to be possible sub-clonal compared with the EGFR mutation, and the correlation between the clonal origin and frequency of detection in plasma was found. In the case of both EGFR and KRAS variant in cfDNA, we can show the presence of KRAS in the tumor tissue variants associated with lack of response to tyrosine kinase inhibitors (TKI) .Although sequencing artifacts can be identified in order cfDNA targets, tumor heterogeneity and CHIP are likely to affect the suitability between plasma and tissue testing.

Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient.

Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient. Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.
Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient. Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.

Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.

Trypan Blue (0.4%)

1209-10
EUR 131

Trypan Blue Trypan Blue Solution, 0.4% Solution in PBS.

TBL02-100ML 100 ml
EUR 82
  • Product line: Animal Cell Culture Media
  • Product family: Buffers and Supplements
Description: Trypan Blue Solution, 0.4% Solution in PBS.

Trypan Blue Assay Kit

AR1175 1kit (for 1000 assays)
EUR 65

Trypan Blue (C.I. 23850)

GT3970-100G 100 g
EUR 110

Trypan Blue (C.I. 23850)

GT3970-25G 25 g
EUR 62

OPTIONAL BLUE FILTER (497NM)

GDBL-BLUE 1/pk
EUR 1082
Description: Lab Equipment; Axygen Branded EQ

conversion screen uv/blue

VLFC26-BLUE ea
EUR 606

Trypan Blue, sodium salt *CAS 72-57-1*

2450 100 g
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Trypan Blue, sodium salt *10 mM aqueous solution*

2453 10 mL
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Cell Counting Kit-8

HY-K0301 120 mL (12000 T)
EUR 1135

Cell Counting Kit (CCK)

20-abx098245
  • EUR 523.00
  • EUR 411.00
  • EUR 718.00
  • EUR 467.00
  • 10 ml
  • 1 ml
  • 30 ml
  • 5 ml
  • Shipped within 5-10 working days.

Trypan red

HY-D0983 5mg
EUR 533

CCK-8 Cell Counting Kit

A311-01 500 rxn
EUR 179

CCK-8 Cell Counting Kit

A311-02 1000 rxn
EUR 234

Trypan Blue, sodium salt *UltraPure grade* *Purified to eliminate fluorescent impurities*

2452 10 g
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Cell Counting Kit-8 (CCK-8)

B34302 5 mL (500 ractions)
EUR 130
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

Cell Counting Kit-8 (CCK-8)

B34304 25 mL (2500 ractions)
EUR 315
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

Cell Counting Kit-8 (CCK-8)

AR1160 5 mL (for 500 assays, 10μL per well)
EUR 131

Cell Counting Kit-8 (CCK-8)

K1018-1 1x1 ml (100 tests)
EUR 73

Cell Counting Kit-8 (CCK-8)

K1018-10 2x5 ml (1000 tests)
EUR 166

Cell Counting Kit-8 (CCK-8)

K1018-100 20x5 ml (10000 tests)
EUR 885

Cell Counting Kit-8 (CCK-8)

K1018-30 6x5 ml (3000 tests)
EUR 328

Cell Counting Kit-8 (CCK-8)

K1018-5 1x5 ml (500 tests)
EUR 119

CORNING COUNTING CHAMBER FOR CORNING CELL COUNTER

480200 1/pk
EUR 142
Description: Lab Equipment; Cell Counters

Cell Counting Kit-8 (CCK8) Cell Viability Assay Kit

abx090677-500tests 500 tests
EUR 394
  • Shipped within 5-10 working days.

Bacterial Counting Colorimetric Assay Kit

K511-2500
EUR 620

Bacterial Counting Colorimetric Assay Kit

K511-500
EUR 349

ECOS Blue (XL1-Blue

FYE107-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE107-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE108-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE108-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE109-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE109-80VL 100 µl x 10 vials Ask for price

Blue Chemically Competent Cell (TetR)

20-abx098068
  • EUR 425.00
  • EUR 453.00
  • 1 ml
  • 2 ml
  • Shipped within 5-10 working days.

Trypan Purple™ *0.1 M aqueous solution*

2465 10 mL
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Trypan Purple™ *0.1 M aqueous solution*

2466 100 mL
EUR 876
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

CellCount-Blue Cell Proliferation Assay Kit

C0100-025 250 Assays
EUR 111

CellCount-Blue Cell Proliferation Assay Kit

C0100-050 500 Assays
EUR 134

CellCount-Blue Cell Proliferation Assay Kit

C0100-100 1000 Assays
EUR 188

CellCount-Blue Cell Proliferation Assay Kit

C0100-500 5000 Assays
EUR 533

Blue Chemically Competent Cell (TetR, CamR)

20-abx098069
  • EUR 439.00
  • EUR 481.00
  • 1 ml
  • 2 ml
  • Shipped within 5-10 working days.

CellQuanti-Blue Cell Viability Assay Kits

CQBL-05K 5000
EUR 221
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 5000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

CellQuanti-Blue Cell Viability Assay Kits

CQBL-10K 10000
EUR 340
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 10000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

Bromophenol Blue

B7774-5000 5 g
EUR 87
Description: IC50: N/ABromophenol blue (3',3",5',5"-tetrabromophenolsulfonphthalein) is widely used in gel loading buffers. Bromophenol blue is experimentally used as acolor marker, apH indicator, and a dye.

Calcein Blue

HY-101887 25mg
EUR 119

Thiazolyl Blue

HY-15924 10mM/1mL
EUR 113

Evans Blue

HY-B1102 100mg
EUR 119

Bromothymol Blue

HY-D0012 1g
EUR 119

Diphenyl Blue

HY-D0970 10mM/1mL
EUR 113

Methylene Blue

HY-14536 100mg
EUR 119

Meldola's blue

GT0259-10G 10 g
EUR 86

Meldola's blue

GT0259-25G 25 g
EUR 134

Meldola's blue

GT0259-50G 50 g
EUR 213

Bromochlorophenol Blue

GT2674-100MG 100 mg
EUR 78

Bromochlorophenol Blue

GT2674-1G 1 g
EUR 174

Bromophenol Blue

GT2874-100G 100 g
EUR 126

Bromophenol Blue

GT2874-10G 10 g
EUR 52

Bromophenol Blue

GT2874-25G 25 g
EUR 66

Bromophenol Blue

GT2874-50G 50 g
EUR 86

Bromophenol Blue

GT2874-5G 5 g
EUR 45

Hydroxynaphthol blue

GT3182-100G 100 g
EUR 237

Hydroxynaphthol blue

GT3182-10G 10 g
EUR 78

Hydroxynaphthol blue

GT3182-25G 25 g
EUR 110

Hydroxynaphthol blue

GT3182-50G 50 g
EUR 166

Bromothymol Blue

GT6837-10G 10 g
EUR 47

Bromothymol Blue

GT6837-25G 25 g
EUR 61

Bromothymol Blue

GT6837-50G 50 g
EUR 86

Bromothymol Blue

GT6837-5G 5 g
EUR 42

Xylenol Blue

GT7988-1G 1 g
EUR 46

Xylenol Blue

GT7988-25G 25 g
EUR 150

Xylenol Blue

GT7988-5G 5 g
EUR 78

Bromoxylenol Blue

GT8506-10G 10 g
EUR 142

Bromoxylenol Blue

GT8506-1G 1 g
EUR 46

Bromoxylenol Blue

GT8506-5G 5 g
EUR 94

Thymol Blue

GT9755-100G 100 g
EUR 150

Thymol Blue

GT9755-10G 10 g
EUR 50

Thymol Blue

GT9755-25G 25 g
EUR 70

Thymol Blue

GT9755-50G 50 g
EUR 94

Thymol Blue

GT9755-5G 5 g
EUR 42

Bromophenol Blue

G114 5.0 g
EUR 102

Evans Blue

20-abx082082
  • EUR 175.00
  • EUR 217.00
  • 1 g
  • 5 g
  • Shipped within 5-10 working days.

Bromphenol Blue

20-abx082184
  • EUR 230.00
  • EUR 189.00
  • 25 g
  • 5 g
  • Shipped within 5-10 working days.

Evans Blue

20-abx082237
  • EUR 189.00
  • EUR 230.00
  • 1 g
  • 5 g
  • Shipped within 5-10 working days.

Bromphenol Blue

20-abx082376
  • EUR 244.00
  • EUR 189.00
  • 25 g
  • 5 g
  • Shipped within 5-10 working days.

Toluidine Blue

abx082425-5g 5 g
EUR 189
  • Shipped within 5-10 working days.

Bromophenol Blue

abx082607-10g 10 g
EUR 203
  • Shipped within 5-10 working days.

Bromophenol blue

abx183916-500ml 500 ml
EUR 230
  • Shipped within 1-2 weeks.

Bromophenol blue

abx183916-5l 5l
EUR 592
  • Shipped within 1-2 weeks.

Evans Blue

abx186080-125g 125g
EUR 453
  • Shipped within 1-2 weeks.

Phthalocyanine blue

20-abx181340
  • EUR 328.00
  • EUR 217.00
  • EUR 746.00
  • 100 g
  • 25 g
  • 500 g
  • Shipped within 1-2 weeks.

Bromophenol Blue

CH019 25 g
EUR 132

Bromophenol Blue

CH020 50 g
EUR 166

Bromophenol Blue

CH021 100 g
EUR 227

Bromophenol blue

BB2230 25g
EUR 69.14
  • Product category: Electrophoresis Related/Tracking Dyes

Trypan Red Plus™ *0.1 M aqueous solution*

2456 10 mL
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Trypan Red Plus™ *0.1 M aqueous solution*

2457 100 mL
EUR 698
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

TER-119 / Erythroid Cell Antibody (CF-Blue)

20-abx200924
  • EUR 453.00
  • EUR 300.00
  • 100 ug
  • 25 ug
  • Shipped within 2-3 weeks.

Cell Explorer™ Live Cell Labeling Kit *Blue Fluorescence*

22606 200 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Cell Meter™ Cell Viability Assay Kit *Blue Fluorescence*

22785 500 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Trypan UltraBlue™, sodium salt *0.1 M aqueous solution*

2455 1 mL
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Brilliant Blue G

C5579-100000 100 g
EUR 135
Description: Coomassie Brilliant Blue G-250 is used for protein staining in SDS-PAGE, Blue Native PAGE, and the Bradford Method, with high band visibility.

Nitrotetrazolium Blue chloride

B5943-1000 1 g
EUR 134
Description: Nitrotetrazolium Blue chloride

Nitrotetrazolium Blue chloride

B5943-5000 5 g
EUR 293
Description: Nitrotetrazolium Blue chloride

Astra Blue Solution

AST125 125 ml
EUR 104

Astra Blue Solution

AST500 500 ml
EUR 183

Bullet Blender Blue

BBX24B 1each
EUR 3911
Description: Homogenizer for 24 samples in microcentrifuge tubes. Includes starter kit of beads, scoop and tubes. with Air Cooling. With CE certification.

Leucomethylene blue (Mesylate)

HY-19948 50mg
EUR 360

Solvent Blue 35

HY-D0516 500mg
EUR 108

Disperse Blue 148

HY-D0569 10mM/1mL
EUR 172

Brilliant Blue FCF

HY-D0915 500mg
EUR 108

Procion Blue HB

HY-D0965 10mM/1mL
EUR 126

Reactive Blue 4

HY-125815 100mg
EUR 108

Acid Blue 29

GT2925-100G 100 g
EUR 118

Acid Blue 29

GT2925-250G 250 g
EUR 181

Acid Blue 29

GT2925-25G 25 g
EUR 70

Sudan Blue II

GT5180-100G 100 g
EUR 126

Sudan Blue II

GT5180-250G 250 g
EUR 213

Sudan Blue II

GT5180-25G 25 g
EUR 70

Disperse Blue 14

GT6746-25G 25 g
EUR 110

Tetrazolium Blue Chloride

GT7170-1G 1 g
EUR 70

Tetrazolium Blue Chloride

GT7170-5G 5 g
EUR 150

Acid Blue 80

GT9286-100G 100 g
EUR 107

Fluorescent PAK Blue

FP-0567-2 2 mL
EUR 182
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Dextran blue 2000

20-abx082076
  • EUR 217.00
  • EUR 356.00
  • 1 g
  • 5 g
  • Shipped within 5-10 working days.

Alcian Blue 8GX

abx082097-1g 1 g
EUR 217
  • Shipped within 5-10 working days.

Alcian Blue 8GX

abx082417-1g 1 g
EUR 203
  • Shipped within 5-10 working days.

Aniline Blue Solution

ABP125 125 ml
EUR 72

Aniline Blue Solution

ABP500 500 ml
EUR 106

Aniline Blue Solution

ABP999 1000 ml
EUR 133

Pigment Blue 29

abx185539-100g 100 g
EUR 370
  • Shipped within 1-2 weeks.

C.I.Food Blue 1

20-abx186397
  • EUR 217.00
  • EUR 565.00
  • EUR 189.00
  • EUR 384.00
  • EUR 175.00
  • 100 g
  • 1 kg
  • 25 g
  • 500 g
  • 5 g
  • Shipped within 1-2 weeks.

Solvent Blue 68

20-abx186561
  • EUR 217.00
  • EUR 704.00
  • EUR 300.00
  • 1 g
  • 25 g
  • 5 g
  • Shipped within 1-2 weeks.

C.I.Var Blue 6

abx180699-25g 25 g
EUR 551
  • Shipped within 1-2 weeks.

C.I.Reactive Blue 19

20-abx181246
  • EUR 509.00
  • EUR 272.00
  • 25 g
  • 5 g
  • Shipped within 1-2 weeks.

C.I.Solvent Blue 36

20-abx181247
  • EUR 425.00
  • EUR 286.00
  • EUR 203.00
  • 100 g
  • 25 g
  • 5 g
  • Shipped within 1-2 weeks.

Amplite™ Blue

11005 25 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Thiolite™ Blue

21507 5 mg
EUR 132
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

CytoTell™ Blue

22251 500 Tests
EUR 132
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

CytoTell™ Blue

22252 1000 Tests
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

LysoBrite™ Blue

22642 500 Tests
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Evans Blue Tetrasodium

2586-1G
EUR 126

DNASE & TOLUIDINE BLUE

D04-103-10kg 10 kg
EUR 9449

DNASE & TOLUIDINE BLUE

D04-103-2Kg 2 Kg
EUR 2093

DNASE & TOLUIDINE BLUE

D04-103-500g 500 g
EUR 605

1L ProtoStain Blue

NAT1112 1L
EUR 137

25G Alcian Blue

NAT1344 25G
EUR 162

25G Methylene Blue

NAT1352 25G
EUR 156

10G Bromophenol Blue

NAT1358 10G
EUR 141

Methylene Blue Solution

MBS125 125 ml
EUR 76

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