Targeted sequencing analysis of cell-free DNA from metastatic non-small-cell lung cancer patients: clinical and biological implications.

Sequencing artifacts, clonal hematopoietic potential mutation indeterminate (CHIP) and the heterogeneity of the tumor has been hypothesized to contribute to the low suitability of tissue and cell-free DNA (cfDNA) with a targeted molecular profiling sequencing.We cfDNA analyzed by targeted sequencing of Hypersaline wastewater may pose a threat to the biological wastewater treatment process. samples from 30 and 77 EGFR-mutant wild-type EGFR lung cancer metastatic non-small cell (mNSCLC) patients. discordant cases solved with digital droplet PCR (ddPCR) .

With cfDNA testing from a healthy donor, we developed an algorithm to recognize the sequencing artifacts. Applying this method to cfDNA of patients mNSCLC, EGFR mutations detected with good sensitivity (76.7%) and specificity (97.4%). In contrast, the sensitivity and specificity for the KRAS variant was 61.5% and 93.8%, respectively. All of EGFR and KRAS variant was detected in the plasma but not in the network is confirmed by ddPCR, thus excluding sequencing artifacts. In a small proportion of cases, KRAS mutations are found in plasma samples was confirmed in tumor tissue showed tumor heterogeneity.

KRAS variant was found to be possible sub-clonal compared with the EGFR mutation, and the correlation between the clonal origin and frequency of detection in plasma was found. In the case of both EGFR and KRAS variant in cfDNA, we can show the presence of KRAS in the tumor tissue variants associated with lack of response to tyrosine kinase inhibitors (TKI) .Although sequencing artifacts can be identified in order cfDNA targets, tumor heterogeneity and CHIP are likely to affect the suitability between plasma and tissue testing.

Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient.

Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient. Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.
Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient. Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.

Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.

Cell Counting Kit-8

DLSM0248 500 Assays Ask for price
Description: Quantitation of viable cell number in proliferation and cytotoxicity assays.

Cell Counting Kit-8

CSK1175 5ml
EUR 0.1

Cell Counting Kit-8

HY-K0301 120 mL (12000 T)
EUR 1362

Cell Counting Kit (CCK)

abx098245-100l 100 µl
EUR 237.5

Cell Counting Kit (CCK)

abx098245-200l 200 µl
EUR 312.5

Cell Counting Kit-8

MBS8305412-100Assays 100Assays
EUR 275

Cell Counting Kit-8

MBS8305412-500Assays 500Assays
EUR 365

Cell Counting Kit-8

MBS8305412-5x500Assays 5x500Assays
EUR 1580

Cell Counting Kit-8

RDSM248 500 Assays
EUR 245
Description: Quantitation of viable cell number in proliferation and cytotoxicity assays.

CCK-8 Cell Counting Kit

A311-01 500 rxn
EUR 51.3

CCK-8 Cell Counting Kit

A311-02 1000 rxn
EUR 87

CCK-8 Cell Counting Kit

A311-00-50rxns 50 rxns
EUR 1.09

CCK-8 Cell Counting Kit

A311-01-500rxns 500 rxns
EUR 53.12

CCK-8 Cell Counting Kit

A311-02-1000rxns 1,000 rxns
EUR 90.09

Trypan Blue Cell Viability Assay

TBS2008 100 mL
EUR 70

Cell Counting Kit-8 (CCK-8)

AR1160 5 mL (for 500 assays, 10μL per well)
EUR 157.2

Cell Counting Kit-8 (CCK-8)

B34302 5 mL (500 ractions)
EUR 156
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

Cell Counting Kit-8 (CCK-8)

B34304 25 mL (2500 ractions)
EUR 378
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

Cell Counting Kit-8 (CCK-8)

C0005-10mg 10mg Ask for price
Description: Cell Counting Kit-8 (CCK-8)

Cell Counting Kit-8 (CCK-8)

C0005-1g 1g Ask for price
Description: Cell Counting Kit-8 (CCK-8)

Cell Counting Kit-8 (CCK-8)

C0005-1mg 1mg Ask for price
Description: Cell Counting Kit-8 (CCK-8)

Cell Counting Kit-8 (CCK-8)

C0005-50mg 50mg Ask for price
Description: Cell Counting Kit-8 (CCK-8)

Cell Counting Kit-8 (CCK-8)

C0005-5mg 5mg Ask for price
Description: Cell Counting Kit-8 (CCK-8)

Cell Counting Kit-8 (CCK-8)

KTA1020-each each Ask for price

Cell Counting Kit-8 (CCK-8)

KTA1020-10000T 10000 T
EUR 549
Description: Economical Cell Counting Kit-8 (CCK-8) is designed for detecting Cell Proliferation and Cytotoxicity

Cell Counting Kit-8 (CCK-8)

KTA1020-1000T 1000 T
EUR 89
Description: Economical Cell Counting Kit-8 (CCK-8) is designed for detecting Cell Proliferation and Cytotoxicity

Cell Counting Kit-8 (CCK-8)

K1018-1 1x1 ml (100 tests)
EUR 16
Description: Used in cell proliferation assay and cytotoxicity assay

Cell Counting Kit-8 (CCK-8)

K1018-10 2x5 ml (1000 tests)
EUR 80
Description: Used in cell proliferation assay and cytotoxicity assay

Cell Counting Kit-8 (CCK-8)

K1018-100 20x5 ml (10000 tests)
EUR 576
Description: Used in cell proliferation assay and cytotoxicity assay

Cell Counting Kit-8 (CCK-8)

K1018-30 6x5 ml (3000 tests)
EUR 192
Description: Used in cell proliferation assay and cytotoxicity assay

Cell Counting Kit-8 (CCK-8)

K1018-5 1x5 ml (500 tests)
EUR 48
Description: Used in cell proliferation assay and cytotoxicity assay

Cell Counting Kit-8 (CCK-8)

MBS8506062-1000Assays 1000Assays
EUR 450

Cell Counting Kit-8 (CCK-8)

MBS8506062-100Assays 100Assays
EUR 200

Cell Counting Kit-8 (CCK-8)

MBS8506062-500Assays 500Assays
EUR 320

Cell Counting Kit-8 (CCK-8)

MBS8506062-5x1000Assays 5x1000Assays
EUR 2050

Cell Counting Kit-8 (CCK-8)

MBS176412-500Tests 500Tests
EUR 175

Cell Counting Kit-8 (CCK-8)

MBS176412-5x500Tests 5x500Tests
EUR 645

Cell Counting Kit-8 (CCK-8)

MBS9719068-1000Tests 1000Tests
EUR 195

Cell Counting Kit-8 (CCK-8)

MBS9719068-10KTests 10KTests
EUR 475

Cell Counting Kit-8 (CCK-8)

MBS9719068-5x10KTests 5x10KTests
EUR 2120

T-Pro Cell Counting Kit (CCK8)

JK95-C008L 5ml*20/BT
EUR 1000

T-Pro Cell Counting Kit (CCK8)

JK95-C008M 5ml*2/BT
EUR 120

T-Pro Cell Counting Kit (CCK8)

JK95-C008S 1ml*5/BT
EUR 80

Cell Counting Pot Square Base - PK200

CEL10500 PK200
EUR 70.2

Trypan Blue

20-abx082476
  • Ask for price
  • Ask for price
  • 25 g
  • 5 g

Trypan Blue

40140044-1 25 g
EUR 22.88

Trypan Blue

40140044-2 100 g
EUR 66.8

Trypan Blue

58026 25 Gms
EUR 7.95
Description: Part B

Trypan Blue

abx082476-100l 100 µl
EUR 137.5

Trypan Blue

abx082476-1ml 1 ml Ask for price

Trypan Blue

abx082476-200l 200 µl
EUR 200

Trypan blue

TT1140 10g
EUR 78.79

Counting Slide 2 Cell Mcmaster - EACH

HAE2030 EACH
EUR 243.82

Trypan Blue Staining Cell Assay Kit

APB0003-10 5ml
EUR 0.1

CORNING COUNTING CHAMBER FOR CORNING CELL COUNTER

480200 1/pk
EUR 170.4
Description: Lab Equipment; Cell Counters

QUANTOM M50 Cell Counting Slides, 50 Slides

LBQ12001 each
EUR 202.74

QUANTOM M50 Cell Counting Slides, 500 Slides

LBQ12002 each
EUR 1640.45

Enhance Cell Counting Kit 8 (WST-8/CCK8)

MBS2567123-1000Tests 1000Tests
EUR 225

Enhance Cell Counting Kit 8 (WST-8/CCK8)

MBS2567123-100Tests 100Tests
EUR 160

Enhance Cell Counting Kit 8 (WST-8/CCK8)

MBS2567123-10KTests 10KTests
EUR 690

Enhance Cell Counting Kit 8 (WST-8/CCK8)

MBS2567123-500Tests 500Tests
EUR 195

Enhance Cell Counting Kit 8 (WST-8/CCK8)

MBS2567123-5x10KTests 5x10KTests
EUR 3160

Cell Counting Slides, 50 slides (100 counts), 1 box

LBL12001 each
EUR 73.03

Enhanced Cell Counting Kit 8 (WST-8/CCK8)

E-CK-A362-10000Tests 10000 Tests
EUR 790

Enhanced Cell Counting Kit 8 (WST-8/CCK8)

E-CK-A362-1000Tests 1000 Tests
EUR 125

Enhanced Cell Counting Kit 8 (WST-8/CCK8)

E-CK-A362-100Tests 100 Tests
EUR 30

Enhanced Cell Counting Kit 8 (WST-8/CCK8)

E-CK-A362-500Tests 500 Tests
EUR 80

Enhanced Cell Counting Kit 8 (WST-8/CCK8)

E-CK-A362-each each Ask for price
Description: Viability/Proliferation/Cytotoxicity

Cell Counting Kit-8 (CCK8) Cell Viability Assay Kit

abx090677-500tests 500 tests
EUR 472.8

Cell Counting Kit-8 (CCK8) Cell Viability Assay Kit

abx090677-100l 100 µl
EUR 187.5

Cell Counting Kit-8 (CCK8) Cell Viability Assay Kit

abx090677-1ml 1 ml Ask for price

Cell Counting Kit-8 (CCK8) Cell Viability Assay Kit

abx090677-200l 200 µl
EUR 262.5

Plastic cell counting slides Kovas Slide - EACH

DD50126 EACH
EUR 330.75

Cell Counting Slides, 500 slides (1000 counts), 10 boxes

LBL12002 each
EUR 648.55

Cell Counting Slides, 1000 slides (2000 counts), 20 boxes

LBL12003 each
EUR 1242.6

Trypan Blue Trypan Blue Solution, 0.4% Solution in PBS.

TBL02-100ML 100 ml
EUR 98.4
Description: Trypan Blue Solution, 0.4% Solution in PBS.

Trypan Blue (0.4%)

1209-10 each
EUR 157.2

Trypan Blue (C.I. 23850)

GT3970 25g
EUR 72.46

Trypan Blue (C.I. 23850)

GT3970-100 100
EUR 79.1

Trypan Blue (C.I. 23850)

GT3970-100G 100 g
EUR 132

Trypan Blue (C.I. 23850)

GT3970-25 25
EUR 31.7

Trypan Blue (C.I. 23850)

GT3970-25G 25 g
EUR 74.4

Trypan Blue (C.I. 23850)

GT0822-GT0822100ml GT0822-100ml
EUR 47.4

SuperKine Maximum Sensitivity Cell Counting Kit-8 (CCK-8)

MBS9719199-1000Tests 1000Tests
EUR 125

SuperKine Maximum Sensitivity Cell Counting Kit-8 (CCK-8)

MBS9719199-200Tests 200Tests
EUR 95

SuperKine Maximum Sensitivity Cell Counting Kit-8 (CCK-8)

MBS9719199-5x1000Tests 5x1000Tests
EUR 515

Trypan Blue Assay Kit

AR1175 1kit (for 1000 assays)
EUR 78

Trypan Blue 0.4% Solution

25561-250 250ml
EUR 56

Trypan Blue 0.4% Solution

40140051-1 100 mL
EUR 12.81

Trypan Blue 0.4% Solution

40140051-2 15 mL
EUR 25.8

Trypan Blue 0.4% Solution

12788 50 ml
EUR 3
Description: Part B

0.4% Trypan Blue Solution

K1183-100 100ml
EUR 64
Description: Cell Biology|Staining Solution

0.4% Trypan Blue Solution

K1183-50 50ml
EUR 40
Description: Cell Biology|Staining Solution

0.4% Trypan Blue Solution

PB180423-100mL 100 mL
EUR 20
Description: Supplements & Reagents

0.4% Trypan Blue Solution

PB180423 100mL
EUR 20
Description: Others

0.4% Trypan Blue solution

MBS2567254-100mL 100mL
EUR 95

0.4% Trypan Blue solution

MBS2567254-5x100mL 5x100mL
EUR 410

SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8)

BMU106-EN-10000T 10000 T
EUR 529
Description: SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (Formazan), which is soluble in the tissue culture medium. The amount of the formazan in cells is directly proportional to the number of living cells

SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8)

BMU106-EN-1000T 1000 T
EUR 69
Description: SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (Formazan), which is soluble in the tissue culture medium. The amount of the formazan in cells is directly proportional to the number of living cells

SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8)

BMU106-EN-200T 200 T
EUR 29
Description: SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (Formazan), which is soluble in the tissue culture medium. The amount of the formazan in cells is directly proportional to the number of living cells

SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8)

BMU106-EN 1000 T Ask for price

Trypan blue, Practical grade

GRM1001-100G 1 unit
EUR 46.58
Description: Trypan blue, Practical grade

Trypan blue, Practical grade

GRM1001-25G 1 unit
EUR 12.5
Description: Trypan blue, Practical grade

Trypan Blue (Technical Grade)

T895523 10g
EUR 131
Description: 72-57-1

Trypan Blue C. I. No. 23850

T32825 100G
EUR 193.7

Trypan blue, Hi-CERT™

GRM263-10G 1 unit
EUR 8.44
Description: Trypan blue, Hi-CERT™

Trypan blue, Hi-CERT™

GRM263-5G 1 unit
EUR 6.93
Description: Trypan blue, Hi-CERT™

Trypan Blue (C.I. 23850); 0.4% solution

GT0822 100ml
EUR 43.43

Nutrient Agar w/ Trypan blue

M2051-100G 1 unit
EUR 14.38
Description: Nutrient Agar w/ Trypan blue

Nutrient Agar w/ Trypan blue

M2051-500G 1 unit
EUR 43.6
Description: Nutrient Agar w/ Trypan blue

0.5%-Trypan Blue Stain Solution

29853-34 100ML
EUR 19.6

Organoid Counting Software - EACH

6749-OC EACH
EUR 7902.9

Trypan Blue for tissue culture

31591 25 Gms
EUR 12.53
Description: Part B

Marker Pen ?for Digital counting

LA660D-1NO 1 unit
EUR 5.34
Description: Marker Pen ?for Digital counting

Coverslip for counting chamber - PK10

Z5000 PK10
EUR 27.17

Counting Chamber Helber Bacteria - EACH

COU9100 EACH
EUR 157.16

100mL Trypan Blue Solution wv - EACH

25-900-CI EACH
EUR 29.7

Bacterial Counting Colorimetric Assay Kit

K511-2500 each
EUR 744

Bacterial Counting Colorimetric Assay Kit

K511-500 each
EUR 418.8

Trypan Blue Stain, 0.4%, Sterile-filtered

LBT13011 each
EUR 45.24

EnoGeneCell™ Counting Kit-8 (CCK-8)

E1CK-000208-1 100 assays
EUR 80

EnoGeneCell™ Counting Kit-8 (CCK-8)

E1CK-000208-10 1000 assays
EUR 350

EnoGeneCell™ Counting Kit-8 (CCK-8)

E1CK-000208-5 500 assays
EUR 215

EnoGeneCell™ Counting Kit-8 (CCK-8)

E1CK-000208-100 10000 assays (100ml)
EUR 350
Description: HOT

EnoGeneCell™ Counting Kit-8 (CCK-8)

E1CK-000208-50 5000 assays (50ml)
EUR 215
Description: HOT

Fast Read Disposable Counting Chambers - PK100

390497 PK100
EUR 220.05

BRAND(TM) BLAUBRAND(R) COUNTING CHAMBER - EACH

BR717820-1EA EACH
EUR 112.05

Trypan Blue, sodium salt *CAS 72-57-1*

2450 100 g
EUR 1089
Description: Trypan blue, a diazo dye is a vital stain that colors dead tissues or cells blue.

Trypan Blue, sodium salt *CAS 72-57-1*

2450-100g 100 g
EUR 1067
Description: Trypan blue, a diazo dye is a vital stain that colors dead tissues or cells blue.

Counting Chamber Improved Neubauer 2 x Bright - EACH

HAE2118 EACH
EUR 129.25

Trypan Blue, sodium salt *10 mM aqueous solution*

2453 10 mL
EUR 227
Description: Trypan blue, a diazo dye is a vital stain that colors dead tissues or cells blue.

Trypan Blue, sodium salt *10 mM aqueous solution*

2453-10mL 10 mL
EUR 222
Description: Trypan blue, a diazo dye is a vital stain that colors dead tissues or cells blue.

Recombinant Dictyostelium discoideum Counting factor 60 (cf60)

MBS1431104-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1265

Recombinant Dictyostelium discoideum Counting factor 60 (cf60)

MBS1431104-002mgEColi 0.02mg(E-Coli)
EUR 935

Recombinant Dictyostelium discoideum Counting factor 60 (cf60)

MBS1431104-002mgYeast 0.02mg(Yeast)
EUR 1060

Recombinant Dictyostelium discoideum Counting factor 60 (cf60)

MBS1431104-01mgEColi 0.1mg(E-Coli)
EUR 1125

Recombinant Dictyostelium discoideum Counting factor 60 (cf60)

MBS1431104-01mgYeast 0.1mg(Yeast)
EUR 1205

Synbiosis AutoCOL Fully Automated Colony Counting System - EACH

COU2020 EACH
EUR 141750

Recombinant Dictyostelium discoideum Counting factor 50 (cf50-1)

MBS1390573-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1160

Recombinant Dictyostelium discoideum Counting factor 50 (cf50-1)

MBS1390573-002mgEColi 0.02mg(E-Coli)
EUR 800

Recombinant Dictyostelium discoideum Counting factor 50 (cf50-1)

MBS1390573-002mgYeast 0.02mg(Yeast)
EUR 950

Recombinant Dictyostelium discoideum Counting factor 50 (cf50-1)

MBS1390573-01mgEColi 0.1mg(E-Coli)
EUR 960

Recombinant Dictyostelium discoideum Counting factor 50 (cf50-1)

MBS1390573-01mgYeast 0.1mg(Yeast)
EUR 1110

Recombinant Dictyostelium discoideum Counting factor 45-1 (cf45-1)

MBS1449880-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1155

Recombinant Dictyostelium discoideum Counting factor 45-1 (cf45-1)

MBS1449880-002mgEColi 0.02mg(E-Coli)
EUR 790

Recombinant Dictyostelium discoideum Counting factor 45-1 (cf45-1)

MBS1449880-002mgYeast 0.02mg(Yeast)
EUR 940

Recombinant Dictyostelium discoideum Counting factor 45-1 (cf45-1)

MBS1449880-01mgEColi 0.1mg(E-Coli)
EUR 950

Recombinant Dictyostelium discoideum Counting factor 45-1 (cf45-1)

MBS1449880-01mgYeast 0.1mg(Yeast)
EUR 1105

Recombinant Dictyostelium discoideum Counting factor-associated protein C (cfaC)

MBS1324507-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Leave a Comment