Sequencing artifacts, clonal hematopoietic potential mutation indeterminate (CHIP) and the heterogeneity of the tumor has been hypothesized to contribute to the low suitability of tissue and cell-free DNA (cfDNA) with a targeted molecular profiling sequencing.We cfDNA analyzed by targeted sequencing of Hypersaline wastewater may pose a threat to the biological wastewater treatment process. samples from 30 and 77 EGFR-mutant wild-type EGFR lung cancer metastatic non-small cell (mNSCLC) patients. discordant cases solved with digital droplet PCR (ddPCR) .
With cfDNA testing from a healthy donor, we developed an algorithm to recognize the sequencing artifacts. Applying this method to cfDNA of patients mNSCLC, EGFR mutations detected with good sensitivity (76.7%) and specificity (97.4%). In contrast, the sensitivity and specificity for the KRAS variant was 61.5% and 93.8%, respectively. All of EGFR and KRAS variant was detected in the plasma but not in the network is confirmed by ddPCR, thus excluding sequencing artifacts. In a small proportion of cases, KRAS mutations are found in plasma samples was confirmed in tumor tissue showed tumor heterogeneity.
KRAS variant was found to be possible sub-clonal compared with the EGFR mutation, and the correlation between the clonal origin and frequency of detection in plasma was found. In the case of both EGFR and KRAS variant in cfDNA, we can show the presence of KRAS in the tumor tissue variants associated with lack of response to tyrosine kinase inhibitors (TKI) .Although sequencing artifacts can be identified in order cfDNA targets, tumor heterogeneity and CHIP are likely to affect the suitability between plasma and tissue testing.
Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient.

Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.
![]() Trypan Blue (0.4%) |
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1209-10 | Biovision | EUR 131.00 |
![]() Trypan Blue Trypan Blue Solution, 0.4% Solution in PBS. |
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TBL02-100ML | Caisson Labs | 100 ml | EUR 82.00 |
|
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Description: Trypan Blue Solution, 0.4% Solution in PBS. |
![]() Trypan Blue Assay Kit |
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AR1175 | BosterBio | 1kit (for 1000 assays) | EUR 65.00 |
![]() Trypan Blue (C.I. 23850) |
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GT3970-100G | Glentham Life Sciences | 100 g | EUR 110.00 |
![]() Trypan Blue (C.I. 23850) |
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GT3970-25G | Glentham Life Sciences | 25 g | EUR 62.00 |
![]() OPTIONAL BLUE FILTER (497NM) |
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GDBL-BLUE | CORNING | 1/pk | EUR 1082.00 |
Description: Lab Equipment; Axygen Branded EQ |
![]() conversion screen uv/blue |
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VLFC26-BLUE | Consort | ea | EUR 606.00 |
![]() Trypan Blue, sodium salt *CAS 72-57-1* |
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2450 | AAT Bioquest | 100 g | EUR 219.00 |
|
![]() Trypan Blue, sodium salt *10 mM aqueous solution* |
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2453 | AAT Bioquest | 10 mL | EUR 219.00 |
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![]() Cell Counting Kit-8 |
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HY-K0301 | MedChemExpress | 120 mL (12000 T) | EUR 1135.00 |
![]() Cell Counting Kit (CCK) |
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20-abx098245 | Abbexa |
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![]() Trypan red |
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HY-D0983 | MedChemExpress | 5mg | EUR 533.00 |
![]() CCK-8 Cell Counting Kit |
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A311-01 | Vazyme | 500 rxn | EUR 179.00 |
![]() CCK-8 Cell Counting Kit |
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A311-02 | Vazyme | 1000 rxn | EUR 234.00 |
![]() Trypan Blue, sodium salt *UltraPure grade* *Purified to eliminate fluorescent impurities* |
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2452 | AAT Bioquest | 10 g | EUR 219.00 |
|
![]() Cell Counting Kit-8 (CCK-8) |
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B34302 | Bimake | 5 mL (500 ractions) | EUR 130.00 |
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading. |
![]() Cell Counting Kit-8 (CCK-8) |
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B34304 | Bimake | 25 mL (2500 ractions) | EUR 315.00 |
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading. |
![]() Cell Counting Kit-8 (CCK-8) |
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AR1160 | BosterBio | 5 mL (for 500 assays, 10μL per well) | EUR 131.00 |
![]() Cell Counting Kit-8 (CCK-8) |
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K1018-1 | ApexBio | 1x1 ml (100 tests) | EUR 73.00 |
![]() Cell Counting Kit-8 (CCK-8) |
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K1018-10 | ApexBio | 2x5 ml (1000 tests) | EUR 166.00 |
![]() Cell Counting Kit-8 (CCK-8) |
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K1018-100 | ApexBio | 20x5 ml (10000 tests) | EUR 885.00 |
![]() Cell Counting Kit-8 (CCK-8) |
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K1018-30 | ApexBio | 6x5 ml (3000 tests) | EUR 328.00 |
![]() Cell Counting Kit-8 (CCK-8) |
|||
K1018-5 | ApexBio | 1x5 ml (500 tests) | EUR 119.00 |
![]() CORNING COUNTING CHAMBER FOR CORNING CELL COUNTER |
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480200 | CORNING | 1/pk | EUR 142.00 |
Description: Lab Equipment; Cell Counters |
![]() Cell Counting Kit-8 (CCK8) Cell Viability Assay Kit |
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abx090677-500tests | Abbexa | 500 tests | EUR 394.00 |
|
![]() Bacterial Counting Colorimetric Assay Kit |
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K511-2500 | Biovision | EUR 620.00 |
![]() Bacterial Counting Colorimetric Assay Kit |
|||
K511-500 | Biovision | EUR 349.00 |
![]() ECOS Blue (XL1-Blue |
|||
FYE107-10VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
![]() ECOS Blue (XL1-Blue |
|||
FYE107-80VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
![]() ECOS Blue (XL1-Blue |
|||
FYE108-10VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
![]() ECOS Blue (XL1-Blue |
|||
FYE108-80VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
![]() ECOS Blue (XL1-Blue |
|||
FYE109-10VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
![]() ECOS Blue (XL1-Blue |
|||
FYE109-80VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
![]() Blue Chemically Competent Cell (TetR) |
|||
20-abx098068 | Abbexa |
|
|
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![]() Trypan Purple™ *0.1 M aqueous solution* |
|||
2465 | AAT Bioquest | 10 mL | EUR 219.00 |
|
![]() Trypan Purple™ *0.1 M aqueous solution* |
|||
2466 | AAT Bioquest | 100 mL | EUR 876.00 |
|
![]() CellCount-Blue Cell Proliferation Assay Kit |
|||
C0100-025 | GenDepot | 250 Assays | EUR 111.00 |
![]() CellCount-Blue Cell Proliferation Assay Kit |
|||
C0100-050 | GenDepot | 500 Assays | EUR 134.00 |
![]() CellCount-Blue Cell Proliferation Assay Kit |
|||
C0100-100 | GenDepot | 1000 Assays | EUR 188.00 |
![]() CellCount-Blue Cell Proliferation Assay Kit |
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C0100-500 | GenDepot | 5000 Assays | EUR 533.00 |
![]() Blue Chemically Competent Cell (TetR, CamR) |
|||
20-abx098069 | Abbexa |
|
|
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![]() CellQuanti-Blue Cell Viability Assay Kits |
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CQBL-05K | BioAssay Systems | 5000 | EUR 221.00 |
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 5000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C. |
![]() CellQuanti-Blue Cell Viability Assay Kits |
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CQBL-10K | BioAssay Systems | 10000 | EUR 340.00 |
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 10000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C. |
![]() Bromophenol Blue |
|||
B7774-5000 | ApexBio | 5 g | EUR 87.00 |
Description: IC50: N/ABromophenol blue (3',3",5',5"-tetrabromophenolsulfonphthalein) is widely used in gel loading buffers. Bromophenol blue is experimentally used as acolor marker, apH indicator, and a dye. |
![]() Calcein Blue |
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HY-101887 | MedChemExpress | 25mg | EUR 119.00 |
![]() Thiazolyl Blue |
|||
HY-15924 | MedChemExpress | 10mM/1mL | EUR 113.00 |
![]() Evans Blue |
|||
HY-B1102 | MedChemExpress | 100mg | EUR 119.00 |
![]() Bromothymol Blue |
|||
HY-D0012 | MedChemExpress | 1g | EUR 119.00 |
![]() Diphenyl Blue |
|||
HY-D0970 | MedChemExpress | 10mM/1mL | EUR 113.00 |
![]() Methylene Blue |
|||
HY-14536 | MedChemExpress | 100mg | EUR 119.00 |
![]() Meldola's blue |
|||
GT0259-10G | Glentham Life Sciences | 10 g | EUR 86.00 |
![]() Meldola's blue |
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GT0259-25G | Glentham Life Sciences | 25 g | EUR 134.00 |
![]() Meldola's blue |
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GT0259-50G | Glentham Life Sciences | 50 g | EUR 213.00 |
![]() Bromochlorophenol Blue |
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GT2674-100MG | Glentham Life Sciences | 100 mg | EUR 78.00 |
![]() Bromochlorophenol Blue |
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GT2674-1G | Glentham Life Sciences | 1 g | EUR 174.00 |
![]() Bromophenol Blue |
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GT2874-100G | Glentham Life Sciences | 100 g | EUR 126.00 |
![]() Bromophenol Blue |
|||
GT2874-10G | Glentham Life Sciences | 10 g | EUR 52.00 |
![]() Bromophenol Blue |
|||
GT2874-25G | Glentham Life Sciences | 25 g | EUR 66.00 |
![]() Bromophenol Blue |
|||
GT2874-50G | Glentham Life Sciences | 50 g | EUR 86.00 |
![]() Bromophenol Blue |
|||
GT2874-5G | Glentham Life Sciences | 5 g | EUR 45.00 |
![]() Hydroxynaphthol blue |
|||
GT3182-100G | Glentham Life Sciences | 100 g | EUR 237.00 |
![]() Hydroxynaphthol blue |
|||
GT3182-10G | Glentham Life Sciences | 10 g | EUR 78.00 |
![]() Hydroxynaphthol blue |
|||
GT3182-25G | Glentham Life Sciences | 25 g | EUR 110.00 |
![]() Hydroxynaphthol blue |
|||
GT3182-50G | Glentham Life Sciences | 50 g | EUR 166.00 |
![]() Bromothymol Blue |
|||
GT6837-10G | Glentham Life Sciences | 10 g | EUR 47.00 |
![]() Bromothymol Blue |
|||
GT6837-25G | Glentham Life Sciences | 25 g | EUR 61.00 |
![]() Bromothymol Blue |
|||
GT6837-50G | Glentham Life Sciences | 50 g | EUR 86.00 |
![]() Bromothymol Blue |
|||
GT6837-5G | Glentham Life Sciences | 5 g | EUR 42.00 |
![]() Xylenol Blue |
|||
GT7988-1G | Glentham Life Sciences | 1 g | EUR 46.00 |
![]() Xylenol Blue |
|||
GT7988-25G | Glentham Life Sciences | 25 g | EUR 150.00 |
![]() Xylenol Blue |
|||
GT7988-5G | Glentham Life Sciences | 5 g | EUR 78.00 |
![]() Bromoxylenol Blue |
|||
GT8506-10G | Glentham Life Sciences | 10 g | EUR 142.00 |
![]() Bromoxylenol Blue |
|||
GT8506-1G | Glentham Life Sciences | 1 g | EUR 46.00 |
![]() Bromoxylenol Blue |
|||
GT8506-5G | Glentham Life Sciences | 5 g | EUR 94.00 |
![]() Thymol Blue |
|||
GT9755-100G | Glentham Life Sciences | 100 g | EUR 150.00 |
![]() Thymol Blue |
|||
GT9755-10G | Glentham Life Sciences | 10 g | EUR 50.00 |
![]() Thymol Blue |
|||
GT9755-25G | Glentham Life Sciences | 25 g | EUR 70.00 |
![]() Thymol Blue |
|||
GT9755-50G | Glentham Life Sciences | 50 g | EUR 94.00 |
![]() Thymol Blue |
|||
GT9755-5G | Glentham Life Sciences | 5 g | EUR 42.00 |
![]() Bromophenol Blue |
|||
G114 | ABM | 5.0 g | EUR 102.00 |
![]() Evans Blue |
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20-abx082082 | Abbexa |
|
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![]() Bromphenol Blue |
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20-abx082184 | Abbexa |
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![]() Evans Blue |
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20-abx082237 | Abbexa |
|
|
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![]() Bromphenol Blue |
|||
20-abx082376 | Abbexa |
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![]() Toluidine Blue |
|||
abx082425-5g | Abbexa | 5 g | EUR 189.00 |
|
![]() Bromophenol Blue |
|||
abx082607-10g | Abbexa | 10 g | EUR 203.00 |
|
![]() Bromophenol blue |
|||
abx183916-500ml | Abbexa | 500 ml | EUR 230.00 |
|
![]() Bromophenol blue |
|||
abx183916-5l | Abbexa | 5l | EUR 592.00 |
|
![]() Evans Blue |
|||
abx186080-125g | Abbexa | 125g | EUR 453.00 |
|
![]() Phthalocyanine blue |
|||
20-abx181340 | Abbexa |
|
|
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![]() Bromophenol Blue |
|||
CH019 | ABM | 25 g | EUR 132.00 |
![]() Bromophenol Blue |
|||
CH020 | ABM | 50 g | EUR 166.00 |
![]() Bromophenol Blue |
|||
CH021 | ABM | 100 g | EUR 227.00 |
![]() Bromophenol blue |
|||
BB2230 | Bio Basic | 25g | EUR 69.14 |
|
![]() Trypan Red Plus™ *0.1 M aqueous solution* |
|||
2456 | AAT Bioquest | 10 mL | EUR 219.00 |
|
![]() Trypan Red Plus™ *0.1 M aqueous solution* |
|||
2457 | AAT Bioquest | 100 mL | EUR 698.00 |
|
![]() TER-119 / Erythroid Cell Antibody (CF-Blue) |
|||
20-abx200924 | Abbexa |
|
|
|
![]() Cell Explorer™ Live Cell Labeling Kit *Blue Fluorescence* |
|||
22606 | AAT Bioquest | 200 Tests | EUR 219.00 |
|
![]() Cell Meter™ Cell Viability Assay Kit *Blue Fluorescence* |
|||
22785 | AAT Bioquest | 500 Tests | EUR 219.00 |
|
![]() Trypan UltraBlue™, sodium salt *0.1 M aqueous solution* |
|||
2455 | AAT Bioquest | 1 mL | EUR 219.00 |
|
![]() Brilliant Blue G |
|||
C5579-100000 | ApexBio | 100 g | EUR 135.00 |
Description: Coomassie Brilliant Blue G-250 is used for protein staining in SDS-PAGE, Blue Native PAGE, and the Bradford Method, with high band visibility. |
![]() Nitrotetrazolium Blue chloride |
|||
B5943-1000 | ApexBio | 1 g | EUR 134.00 |
Description: Nitrotetrazolium Blue chloride |
![]() Nitrotetrazolium Blue chloride |
|||
B5943-5000 | ApexBio | 5 g | EUR 293.00 |
Description: Nitrotetrazolium Blue chloride |
![]() Astra Blue Solution |
|||
AST125 | ScyTek Laboratories | 125 ml | EUR 104.00 |
![]() Astra Blue Solution |
|||
AST500 | ScyTek Laboratories | 500 ml | EUR 183.00 |
![]() Bullet Blender Blue |
|||
BBX24B | Next Advance | 1each | EUR 3911.00 |
Description: Homogenizer for 24 samples in microcentrifuge tubes. Includes starter kit of beads, scoop and tubes. with Air Cooling. With CE certification. |
![]() Leucomethylene blue (Mesylate) |
|||
HY-19948 | MedChemExpress | 50mg | EUR 360.00 |
![]() Solvent Blue 35 |
|||
HY-D0516 | MedChemExpress | 500mg | EUR 108.00 |
![]() Disperse Blue 148 |
|||
HY-D0569 | MedChemExpress | 10mM/1mL | EUR 172.00 |
![]() Brilliant Blue FCF |
|||
HY-D0915 | MedChemExpress | 500mg | EUR 108.00 |
![]() Procion Blue HB |
|||
HY-D0965 | MedChemExpress | 10mM/1mL | EUR 126.00 |
![]() Reactive Blue 4 |
|||
HY-125815 | MedChemExpress | 100mg | EUR 108.00 |
![]() Acid Blue 29 |
|||
GT2925-100G | Glentham Life Sciences | 100 g | EUR 118.00 |
![]() Acid Blue 29 |
|||
GT2925-250G | Glentham Life Sciences | 250 g | EUR 181.00 |
![]() Acid Blue 29 |
|||
GT2925-25G | Glentham Life Sciences | 25 g | EUR 70.00 |
![]() Sudan Blue II |
|||
GT5180-100G | Glentham Life Sciences | 100 g | EUR 126.00 |
![]() Sudan Blue II |
|||
GT5180-250G | Glentham Life Sciences | 250 g | EUR 213.00 |
![]() Sudan Blue II |
|||
GT5180-25G | Glentham Life Sciences | 25 g | EUR 70.00 |
![]() Disperse Blue 14 |
|||
GT6746-25G | Glentham Life Sciences | 25 g | EUR 110.00 |
![]() Tetrazolium Blue Chloride |
|||
GT7170-1G | Glentham Life Sciences | 1 g | EUR 70.00 |
![]() Tetrazolium Blue Chloride |
|||
GT7170-5G | Glentham Life Sciences | 5 g | EUR 150.00 |
![]() Acid Blue 80 |
|||
GT9286-100G | Glentham Life Sciences | 100 g | EUR 107.00 |
![]() Fluorescent PAK Blue |
|||
FP-0567-2 | Spherotech | 2 mL | EUR 182.00 |
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
![]() Dextran blue 2000 |
|||
20-abx082076 | Abbexa |
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|
|
![]() Alcian Blue 8GX |
|||
abx082097-1g | Abbexa | 1 g | EUR 217.00 |
|
![]() Alcian Blue 8GX |
|||
abx082417-1g | Abbexa | 1 g | EUR 203.00 |
|
![]() Aniline Blue Solution |
|||
ABP125 | ScyTek Laboratories | 125 ml | EUR 72.00 |
![]() Aniline Blue Solution |
|||
ABP500 | ScyTek Laboratories | 500 ml | EUR 106.00 |
![]() Aniline Blue Solution |
|||
ABP999 | ScyTek Laboratories | 1000 ml | EUR 133.00 |
![]() Pigment Blue 29 |
|||
abx185539-100g | Abbexa | 100 g | EUR 370.00 |
|
![]() C.I.Food Blue 1 |
|||
20-abx186397 | Abbexa |
|
|
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![]() Solvent Blue 68 |
|||
20-abx186561 | Abbexa |
|
|
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![]() C.I.Var Blue 6 |
|||
abx180699-25g | Abbexa | 25 g | EUR 551.00 |
|
![]() C.I.Reactive Blue 19 |
|||
20-abx181246 | Abbexa |
|
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![]() C.I.Solvent Blue 36 |
|||
20-abx181247 | Abbexa |
|
|
|
![]() Amplite™ Blue |
|||
11005 | AAT Bioquest | 25 mg | EUR 176.00 |
|
![]() Thiolite™ Blue |
|||
21507 | AAT Bioquest | 5 mg | EUR 132.00 |
|
![]() CytoTell™ Blue |
|||
22251 | AAT Bioquest | 500 Tests | EUR 132.00 |
|
![]() CytoTell™ Blue |
|||
22252 | AAT Bioquest | 1000 Tests | EUR 176.00 |
|
![]() LysoBrite™ Blue |
|||
22642 | AAT Bioquest | 500 Tests | EUR 176.00 |
|
![]() Evans Blue Tetrasodium |
|||
2586-1G | Biovision | EUR 126.00 |
![]() DNASE & TOLUIDINE BLUE |
|||
D04-103-10kg | Alphabiosciences | 10 kg | EUR 9449.00 |
![]() DNASE & TOLUIDINE BLUE |
|||
D04-103-2Kg | Alphabiosciences | 2 Kg | EUR 2093.00 |
![]() DNASE & TOLUIDINE BLUE |
|||
D04-103-500g | Alphabiosciences | 500 g | EUR 605.00 |
![]() 1L ProtoStain Blue |
|||
NAT1112 | National Diagnostics | 1L | EUR 137.00 |
![]() 25G Alcian Blue |
|||
NAT1344 | National Diagnostics | 25G | EUR 162.00 |
![]() 25G Methylene Blue |
|||
NAT1352 | National Diagnostics | 25G | EUR 156.00 |
![]() 10G Bromophenol Blue |
|||
NAT1358 | National Diagnostics | 10G | EUR 141.00 |
![]() Methylene Blue Solution |
|||
MBS125 | ScyTek Laboratories | 125 ml | EUR 76.00 |