Targeted sequencing analysis of cell-free DNA from metastatic non-small-cell lung cancer patients: clinical and biological implications.

Sequencing artifacts, clonal hematopoietic potential mutation indeterminate (CHIP) and the heterogeneity of the tumor has been hypothesized to contribute to the low suitability of tissue and cell-free DNA (cfDNA) with a targeted molecular profiling sequencing.We cfDNA analyzed by targeted sequencing of Hypersaline wastewater may pose a threat to the biological wastewater treatment process. samples from 30 and 77 EGFR-mutant wild-type EGFR lung cancer metastatic non-small cell (mNSCLC) patients. discordant cases solved with digital droplet PCR (ddPCR) .

With cfDNA testing from a healthy donor, we developed an algorithm to recognize the sequencing artifacts. Applying this method to cfDNA of patients mNSCLC, EGFR mutations detected with good sensitivity (76.7%) and specificity (97.4%). In contrast, the sensitivity and specificity for the KRAS variant was 61.5% and 93.8%, respectively. All of EGFR and KRAS variant was detected in the plasma but not in the network is confirmed by ddPCR, thus excluding sequencing artifacts. In a small proportion of cases, KRAS mutations are found in plasma samples was confirmed in tumor tissue showed tumor heterogeneity.

KRAS variant was found to be possible sub-clonal compared with the EGFR mutation, and the correlation between the clonal origin and frequency of detection in plasma was found. In the case of both EGFR and KRAS variant in cfDNA, we can show the presence of KRAS in the tumor tissue variants associated with lack of response to tyrosine kinase inhibitors (TKI) .Although sequencing artifacts can be identified in order cfDNA targets, tumor heterogeneity and CHIP are likely to affect the suitability between plasma and tissue testing.

Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient.

Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient. Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.
Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient. Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.

Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.

Trypan Blue (0.4%)

1209-10
EUR 131.00

Trypan Blue Trypan Blue Solution, 0.4% Solution in PBS.

TBL02-100ML 100 ml
EUR 82.00
  • Product line: Animal Cell Culture Media
  • Product family: Buffers and Supplements
Description: Trypan Blue Solution, 0.4% Solution in PBS.

Trypan Blue Assay Kit

AR1175 1kit (for 1000 assays)
EUR 65.00

Trypan Blue (C.I. 23850)

GT3970-100G 100 g
EUR 110.00

Trypan Blue (C.I. 23850)

GT3970-25G 25 g
EUR 62.00

OPTIONAL BLUE FILTER (497NM)

GDBL-BLUE 1/pk
EUR 1082.00
Description: Lab Equipment; Axygen Branded EQ

conversion screen uv/blue

VLFC26-BLUE ea
EUR 606.00

Trypan Blue, sodium salt *CAS 72-57-1*

2450 100 g
EUR 219.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Trypan Blue, sodium salt *10 mM aqueous solution*

2453 10 mL
EUR 219.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Cell Counting Kit (CCK)

20-abx098245
  • EUR 523.00
  • EUR 411.00
  • EUR 718.00
  • EUR 467.00
  • 0
  • 1
  • 2
  • 3
  • Shipped within 5-10 working days.

Cell Counting Kit-8

HY-K0301 120 mL (12000 T)
EUR 1135.00

Trypan red

HY-D0983 5mg
EUR 533.00

CCK-8 Cell Counting Kit

A311-01 500 rxn
EUR 179.00

CCK-8 Cell Counting Kit

A311-02 1000 rxn
EUR 234.00

Trypan Blue, sodium salt *UltraPure grade* *Purified to eliminate fluorescent impurities*

2452 10 g
EUR 219.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Cell Counting Kit-8 (CCK-8)

B34302 5 mL (500 ractions)
EUR 130.00
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

Cell Counting Kit-8 (CCK-8)

B34304 25 mL (2500 ractions)
EUR 315.00
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

Cell Counting Kit-8 (CCK-8)

AR1160 5 mL (for 500 assays, 10μL per well)
EUR 131.00

Cell Counting Kit-8 (CCK-8)

K1018-1 1x1 ml (100 tests)
EUR 73.00

Cell Counting Kit-8 (CCK-8)

K1018-10 2x5 ml (1000 tests)
EUR 166.00

Cell Counting Kit-8 (CCK-8)

K1018-100 20x5 ml (10000 tests)
EUR 885.00

Cell Counting Kit-8 (CCK-8)

K1018-30 6x5 ml (3000 tests)
EUR 328.00

Cell Counting Kit-8 (CCK-8)

K1018-5 1x5 ml (500 tests)
EUR 119.00

CORNING COUNTING CHAMBER FOR CORNING CELL COUNTER

480200 1/pk
EUR 142.00
Description: Lab Equipment; Cell Counters

Cell Counting Kit-8 (CCK8) Cell Viability Assay Kit

abx090677-500tests 500 tests
EUR 394.00
  • Shipped within 5-10 working days.

Bacterial Counting Colorimetric Assay Kit

K511-2500
EUR 620.00

Bacterial Counting Colorimetric Assay Kit

K511-500
EUR 349.00

ECOS Blue (XL1-Blue

FYE107-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE107-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE108-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE108-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE109-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE109-80VL 100 µl x 10 vials Ask for price

Blue Chemically Competent Cell (TetR)

20-abx098068
  • EUR 425.00
  • EUR 453.00
  • 0
  • 1
  • Shipped within 5-10 working days.

Trypan Purple™ *0.1 M aqueous solution*

2465 10 mL
EUR 219.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Trypan Purple™ *0.1 M aqueous solution*

2466 100 mL
EUR 876.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Blue Chemically Competent Cell (TetR, CamR)

20-abx098069
  • EUR 439.00
  • EUR 481.00
  • 0
  • 1
  • Shipped within 5-10 working days.

CellQuanti-Blue Cell Viability Assay Kits

CQBL-05K 5000
EUR 221.00
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 5000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

CellQuanti-Blue Cell Viability Assay Kits

CQBL-10K 10000
EUR 340.00
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 10000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

CellCount-Blue Cell Proliferation Assay Kit

C0100-025 250 Assays
EUR 111.00

CellCount-Blue Cell Proliferation Assay Kit

C0100-050 500 Assays
EUR 134.00

CellCount-Blue Cell Proliferation Assay Kit

C0100-100 1000 Assays
EUR 188.00

CellCount-Blue Cell Proliferation Assay Kit

C0100-500 5000 Assays
EUR 533.00

Phthalocyanine blue

20-abx181340
  • EUR 328.00
  • EUR 217.00
  • EUR 746.00
  • 0
  • 1
  • 2
  • Shipped within 1-2 weeks.

Evans Blue

abx186080-125g 125g
EUR 453.00
  • Shipped within 1-2 weeks.

Bromophenol blue

abx183916-500ml 500 ml
EUR 230.00
  • Shipped within 1-2 weeks.

Bromophenol blue

abx183916-5l 5l
EUR 592.00
  • Shipped within 1-2 weeks.

Evans Blue

20-abx082082
  • EUR 175.00
  • EUR 217.00
  • 0
  • 1
  • Shipped within 5-10 working days.

Bromphenol Blue

20-abx082184
  • EUR 230.00
  • EUR 189.00
  • 0
  • 1
  • Shipped within 5-10 working days.

Evans Blue

20-abx082237
  • EUR 189.00
  • EUR 230.00
  • 0
  • 1
  • Shipped within 5-10 working days.

Bromphenol Blue

20-abx082376
  • EUR 244.00
  • EUR 189.00
  • 0
  • 1
  • Shipped within 5-10 working days.

Toluidine Blue

abx082425-5g 5 g
EUR 189.00
  • Shipped within 5-10 working days.

Bromophenol Blue

abx082607-10g 10 g
EUR 203.00
  • Shipped within 5-10 working days.

Bromophenol Blue

CH019 25 g
EUR 132.00

Bromophenol Blue

CH020 50 g
EUR 166.00

Bromophenol Blue

CH021 100 g
EUR 227.00

Bromophenol Blue

B7774-5000 5 g
EUR 87.00
Description: IC50: N/ABromophenol blue (3',3",5',5"-tetrabromophenolsulfonphthalein) is widely used in gel loading buffers. Bromophenol blue is experimentally used as acolor marker, apH indicator, and a dye.

Bromophenol blue

BB2230 25g
EUR 69.14
  • Product category: Electrophoresis Related/Tracking Dyes

Methylene Blue

HY-14536 100mg
EUR 119.00

Thiazolyl Blue

HY-15924 10mM/1mL
EUR 113.00

Evans Blue

HY-B1102 100mg
EUR 119.00

Bromothymol Blue

HY-D0012 1g
EUR 119.00

Diphenyl Blue

HY-D0970 10mM/1mL
EUR 113.00

Calcein Blue

HY-101887 25mg
EUR 119.00

Bromochlorophenol Blue

GT2674-100MG 100 mg
EUR 78.00

Bromochlorophenol Blue

GT2674-1G 1 g
EUR 174.00

Bromophenol Blue

GT2874-100G 100 g
EUR 126.00

Bromophenol Blue

GT2874-10G 10 g
EUR 52.00

Bromophenol Blue

GT2874-25G 25 g
EUR 66.00

Bromophenol Blue

GT2874-50G 50 g
EUR 86.00

Bromophenol Blue

GT2874-5G 5 g
EUR 45.00

Hydroxynaphthol blue

GT3182-100G 100 g
EUR 237.00

Hydroxynaphthol blue

GT3182-10G 10 g
EUR 78.00

Hydroxynaphthol blue

GT3182-25G 25 g
EUR 110.00

Hydroxynaphthol blue

GT3182-50G 50 g
EUR 166.00

Bromothymol Blue

GT6837-10G 10 g
EUR 47.00

Bromothymol Blue

GT6837-25G 25 g
EUR 61.00

Bromothymol Blue

GT6837-50G 50 g
EUR 86.00

Bromothymol Blue

GT6837-5G 5 g
EUR 42.00

Xylenol Blue

GT7988-1G 1 g
EUR 46.00

Xylenol Blue

GT7988-25G 25 g
EUR 150.00

Xylenol Blue

GT7988-5G 5 g
EUR 78.00

Bromoxylenol Blue

GT8506-10G 10 g
EUR 142.00

Bromoxylenol Blue

GT8506-1G 1 g
EUR 46.00

Bromoxylenol Blue

GT8506-5G 5 g
EUR 94.00

Thymol Blue

GT9755-100G 100 g
EUR 150.00

Thymol Blue

GT9755-10G 10 g
EUR 50.00

Thymol Blue

GT9755-25G 25 g
EUR 70.00

Thymol Blue

GT9755-50G 50 g
EUR 94.00

Thymol Blue

GT9755-5G 5 g
EUR 42.00

Bromophenol Blue

G114 5.0 g
EUR 102.00

Meldola's blue

GT0259-10G 10 g
EUR 86.00

Meldola's blue

GT0259-25G 25 g
EUR 134.00

Meldola's blue

GT0259-50G 50 g
EUR 213.00

Trypan Red Plus™ *0.1 M aqueous solution*

2456 10 mL
EUR 219.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Trypan Red Plus™ *0.1 M aqueous solution*

2457 100 mL
EUR 698.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

TER-119 / Erythroid Cell Antibody (CF-Blue)

20-abx200924
  • EUR 453.00
  • EUR 300.00
  • 0
  • 1
  • Shipped within 2-3 weeks.

Cell Explorer™ Live Cell Labeling Kit *Blue Fluorescence*

22606 200 Tests
EUR 219.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Cell Meter™ Cell Viability Assay Kit *Blue Fluorescence*

22785 500 Tests
EUR 219.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Trypan UltraBlue™, sodium salt *0.1 M aqueous solution*

2455 1 mL
EUR 219.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

CytoTell™ Blue

22251 500 Tests
EUR 132.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

CytoTell™ Blue

22252 1000 Tests
EUR 176.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

LysoBrite™ Blue

22642 500 Tests
EUR 176.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Evans Blue Tetrasodium

2586-1G
EUR 126.00

Thiolite™ Blue

21507 5 mg
EUR 132.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Amplite™ Blue

11005 25 mg
EUR 176.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Nitrotetrazolium Blue chloride

B5943-1000 1 g
EUR 134.00
Description: Nitrotetrazolium Blue chloride

Nitrotetrazolium Blue chloride

B5943-5000 5 g
EUR 293.00
Description: Nitrotetrazolium Blue chloride

Astra Blue Solution

AST125 125 ml
EUR 104.00

Astra Blue Solution

AST500 500 ml
EUR 183.00

DNASE & TOLUIDINE BLUE

D04-103-10kg 10 kg
EUR 9449.00

DNASE & TOLUIDINE BLUE

D04-103-2Kg 2 Kg
EUR 2093.00

DNASE & TOLUIDINE BLUE

D04-103-500g 500 g
EUR 605.00

Alcian blue 8GX

A0298 5g
EUR 70.88
  • Product category: Biochemicals/Indicators/Stains/Biological Stains

C.I.Var Blue 6

abx180699-25g 25 g
EUR 551.00
  • Shipped within 1-2 weeks.

C.I.Reactive Blue 19

20-abx181246
  • EUR 509.00
  • EUR 272.00
  • 0
  • 1
  • Shipped within 1-2 weeks.

C.I.Solvent Blue 36

20-abx181247
  • EUR 425.00
  • EUR 286.00
  • EUR 203.00
  • 0
  • 1
  • 2
  • Shipped within 1-2 weeks.

Pigment Blue 29

abx185539-100g 100 g
EUR 370.00
  • Shipped within 1-2 weeks.

C.I.Food Blue 1

20-abx186397
  • EUR 217.00
  • EUR 565.00
  • EUR 189.00
  • EUR 384.00
  • EUR 175.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 1-2 weeks.

Solvent Blue 68

20-abx186561
  • EUR 217.00
  • EUR 704.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • Shipped within 1-2 weeks.

Dextran blue 2000

20-abx082076
  • EUR 217.00
  • EUR 356.00
  • 0
  • 1
  • Shipped within 5-10 working days.

Alcian Blue 8GX

abx082097-1g 1 g
EUR 217.00
  • Shipped within 5-10 working days.

Alcian Blue 8GX

abx082417-1g 1 g
EUR 203.00
  • Shipped within 5-10 working days.

Brilliant Blue G

C5579-100000 100 g
EUR 135.00
Description: Coomassie Brilliant Blue G-250 is used for protein staining in SDS-PAGE, Blue Native PAGE, and the Bradford Method, with high band visibility.

Blue Gravity Column

BSP089 1Column, 1 UNIT
EUR 64.79
  • Product category: Labware/Columns/Gravity

Bullet Blender Blue

BBX24B 1each
EUR 3911.00
Description: Homogenizer for 24 samples in microcentrifuge tubes. Includes starter kit of beads, scoop and tubes. with Air Cooling. With CE certification.

Aniline Blue Solution

ABP125 125 ml
EUR 72.00

Aniline Blue Solution

ABP500 500 ml
EUR 106.00

Alkali blue 6B

AB0016 25g
EUR 67.40
  • Product category: Biochemicals/Indicators/Stains/Biological Stains

Aniline Blue Solution

ABP999 1000 ml
EUR 133.00

Reactive Blue 4

HY-125815 100mg
EUR 108.00

Leucomethylene blue (Mesylate)

HY-19948 50mg
EUR 360.00

Solvent Blue 35

HY-D0516 500mg
EUR 108.00

Disperse Blue 148

HY-D0569 10mM/1mL
EUR 172.00

Brilliant Blue FCF

HY-D0915 500mg
EUR 108.00

Procion Blue HB

HY-D0965 10mM/1mL
EUR 126.00

Acid Blue 29

GT2925-100G 100 g
EUR 118.00

Acid Blue 29

GT2925-250G 250 g
EUR 181.00

Acid Blue 29

GT2925-25G 25 g
EUR 70.00

Sudan Blue II

GT5180-100G 100 g
EUR 126.00

Sudan Blue II

GT5180-250G 250 g
EUR 213.00

Sudan Blue II

GT5180-25G 25 g
EUR 70.00

Disperse Blue 14

GT6746-25G 25 g
EUR 110.00

Tetrazolium Blue Chloride

GT7170-1G 1 g
EUR 70.00

Tetrazolium Blue Chloride

GT7170-5G 5 g
EUR 150.00

Acid Blue 80

GT9286-100G 100 g
EUR 107.00

Fluorescent PAK Blue

FP-0567-2 2 mL
EUR 182.00
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

1L ProtoStain Blue

NAT1112 1L
EUR 137.00

25G Alcian Blue

NAT1344 25G
EUR 162.00

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