Targeted sequencing analysis of cell-free DNA from metastatic non-small-cell lung cancer patients: clinical and biological implications.

Sequencing artifacts, clonal hematopoietic potential mutation indeterminate (CHIP) and the heterogeneity of the tumor has been hypothesized to contribute to the low suitability of tissue and cell-free DNA (cfDNA) with a targeted molecular profiling sequencing.We cfDNA analyzed by targeted sequencing of Hypersaline wastewater may pose a threat to the biological wastewater treatment process. samples from 30 and 77 EGFR-mutant wild-type EGFR lung cancer metastatic non-small cell (mNSCLC) patients. discordant cases solved with digital droplet PCR (ddPCR) .

With cfDNA testing from a healthy donor, we developed an algorithm to recognize the sequencing artifacts. Applying this method to cfDNA of patients mNSCLC, EGFR mutations detected with good sensitivity (76.7%) and specificity (97.4%). In contrast, the sensitivity and specificity for the KRAS variant was 61.5% and 93.8%, respectively. All of EGFR and KRAS variant was detected in the plasma but not in the network is confirmed by ddPCR, thus excluding sequencing artifacts. In a small proportion of cases, KRAS mutations are found in plasma samples was confirmed in tumor tissue showed tumor heterogeneity.

KRAS variant was found to be possible sub-clonal compared with the EGFR mutation, and the correlation between the clonal origin and frequency of detection in plasma was found. In the case of both EGFR and KRAS variant in cfDNA, we can show the presence of KRAS in the tumor tissue variants associated with lack of response to tyrosine kinase inhibitors (TKI) .Although sequencing artifacts can be identified in order cfDNA targets, tumor heterogeneity and CHIP are likely to affect the suitability between plasma and tissue testing.

Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient.

Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient. Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.
Hypersaline wastewater may pose a threat to the biological wastewater treatment process. Reactor-based aerobic granular sludge sequencing batch (SBR) perform simultaneous nitrification, denitrification and removal of phosphorus (SNDPR) was evaluated with an increase in salinity of 1 to 2% (w / v). nitrogen deletion performance is not affected by the salinity of up to 20 g / L in terms of nitrification and denitrification are reliable and efficient. Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.

Enhanced biological elimination of phosphorus (EBPR) process actually worsened in the salinity of up to 2%, in contrast with the removal of phosphorus excellent at 1%. Profile phosphorus over one cycle shows that the higher salinity not only inhibits anaerobic phosphorus release, but also hampered the aerobic / anoxic phosphorus uptake.

Trypan Blue

20-abx082476
  • EUR 276.00
  • EUR 226.80
  • 25 g
  • 5 g

Trypan blue

TT1140 10g
EUR 78.79

Cell Counting Pot Square Base

CEL10500 PK200
EUR 62.4

Cell Counting Kit-8 (CCK-8)

AR1160 5 mL (for 500 assays, 10μL per well)
EUR 157.2

Cell Counting Kit-8 (CCK-8)

B34302 5 mL (500 ractions)
EUR 156
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

Cell Counting Kit-8 (CCK-8)

B34304 25 mL (2500 ractions)
EUR 378
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

Cell Counting Kit-8 (CCK-8)

K1018-1 1x1 ml (100 tests)
EUR 87.6

Cell Counting Kit-8 (CCK-8)

K1018-10 2x5 ml (1000 tests)
EUR 199.2

Cell Counting Kit-8 (CCK-8)

K1018-100 20x5 ml (10000 tests)
EUR 1062

Cell Counting Kit-8 (CCK-8)

K1018-30 6x5 ml (3000 tests)
EUR 393.6

Cell Counting Kit-8 (CCK-8)

K1018-5 1x5 ml (500 tests)
EUR 142.8

Counting Slide 2 Cell Mcmaster

HAE2030 EACH
EUR 216.73

Trypan Blue (0.4%)

1209-10 each
EUR 157.2

Trypan Blue Trypan Blue Solution, 0.4% Solution in PBS.

TBL02-100ML 100 ml
EUR 98.4
Description: Trypan Blue Solution, 0.4% Solution in PBS.

Plastic cell counting slides Kovas Slide

DD50126 EACH
EUR 294

Trypan Blue (C.I. 23850)

GT3970-100G 100 g
EUR 132

Trypan Blue (C.I. 23850)

GT3970-25G 25 g
EUR 74.4

CORNING COUNTING CHAMBER FOR CORNING CELL COUNTER

480200 1/pk
EUR 170.4
Description: Lab Equipment; Cell Counters

Cell Counting Kit-8 (CCK8) Cell Viability Assay Kit

abx090677-500tests 500 tests
EUR 472.8

Trypan Blue Assay Kit

AR1175 1kit (for 1000 assays)
EUR 78

Organoid Counting Software

6749-OC EACH
EUR 7024.8

Coverslip for counting chamber

Z5000 PK10
EUR 24.15

Counting Chamber Helber Bacteria

COU9100 EACH
EUR 139.7

100mL Trypan Blue Solution wv

25-900-CI EACH
EUR 26.4

Fast Read Disposable Counting Chambers

390497 PK100
EUR 195.6

Bacterial Counting Colorimetric Assay Kit

K511-2500 each
EUR 744

Bacterial Counting Colorimetric Assay Kit

K511-500 each
EUR 418.8

BRAND(TM) BLAUBRAND(R) COUNTING CHAMBER

BR717820-1EA EACH
EUR 99.6

Counting Chamber Improved Neubauer 2 x Bright

HAE2118 EACH
EUR 114.89

Trypan Blue, sodium salt *CAS 72-57-1*

2450 100 g
EUR 1067

Synbiosis AutoCOL Fully Automated Colony Counting System

COU2020 EACH
EUR 126000

Trypan Blue, sodium salt *10 mM aqueous solution*

2453 10 mL
EUR 222

Trypan Blue, sodium salt *UltraPure grade* *Purified to eliminate fluorescent impurities*

2452 10 g
EUR 222

Trypan red

HY-D0983 5mg
EUR 639.6

Evans Blue counter stain/diluent block

85-E09 3 ml
EUR 253.2
Description: Evans Blue counter stain/diluent block for use in immunofluorescent staining

Trypan Purple™ *0.1 M aqueous solution*

2465 10 mL
EUR 222

Trypan Purple™ *0.1 M aqueous solution*

2466 100 mL
EUR 1070

Trypan Red Plus™ *0.1 M aqueous solution*

2456 10 mL
EUR 222

Trypan Red Plus™ *0.1 M aqueous solution*

2457 100 mL
EUR 841

Trypan UltraBlue™, sodium salt *0.1 M aqueous solution*

2455 1 mL
EUR 222

Cyto-Blue, Cytoplasmic counterstain for countersta

NB310 10 ml
EUR 139
Description: Cyto-Blue, Cytoplasmic counterstain for counterstaining nuclear IHC & ICC stains such as ER, PR, Ki-67 and all antibodies staining nuclear markers, 10 ml (Small volume)

Cyto-Blue, Cytoplasmic counterstain for countersta

NB354 100 ml
EUR 355
Description: Cyto-Blue, Cytoplasmic counterstain for counterstaining nuclear IHC& ICC stains such as ER, PR, Ki-67 and all antibodies staining nuclear markers, 100 ml (Large volume)

Cell Counter

6749 EACH
EUR 4480.8

Blue Chemically Competent Cell (TetR)

20-abx098068
  • EUR 510.00
  • EUR 543.60
  • 1 ml
  • 2 ml

Blue Chemically Competent Cell (TetR, CamR)

20-abx098069
  • EUR 526.80
  • EUR 577.20
  • 1 ml
  • 2 ml

Cell Meter™ Cell Viability Assay Kit *Blue Fluorescence*

22785 500 Tests
EUR 222

Cell Explorer™ Live Cell Labeling Kit *Blue Fluorescence*

22606 200 Tests
EUR 222

Cell Explorer™ Live Cell Tracking Kit *Blue Fluorescence*

22620 200 Tests
EUR 222

Cell Strainer 40um Blue Sterile Ind. Wrapped

ARG1218 PK50
EUR 212.4

ECOS Blue (XL1-Blue

FYE107-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE107-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE108-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE108-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE109-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue

FYE109-80VL 100 µl x 10 vials Ask for price

QuadCount™ Automated Cell Counter, 115V

E7500 1 each
EUR 4151.3

QuadCount™ Automated Cell Counter, 230V

E7500-E 1 each
EUR 4151.3

Cell Meter™ Autophagy Assay Kit *Blue Fluorescence*

23000 200 Tests
EUR 222

Accuris QuadCount Automated Cell Counter 230V

COU1060 EACH
EUR 5416.8

Accuris QuadSlides for QuadCount Cell Counter

COU1062 PK50
EUR 223.2

SCOTT extra couch covers blue

CLE7044 12ROLL
EUR 377.34

Snap Cap 11mm Blue Si/ PTFE Blue

CHR3550 PK1000
EUR 1298.4

Snap Cap 11mm Blue Si/PTFE Blue

CHR3552 PK1000
EUR 561.6

Cell Navigator® Lysosome Staining Kit *Blue Fluorescence*

22655 500 Tests
EUR 166

QuadSlides for QuadCount Cell Counter, pack of 50

E7500-S1 1 each
EUR 167.4

Container 125ml PP Blue+Label+Blue Cap

CON1013 PK380
EUR 241.2

Evans Blue

abx186080-125g 125g
EUR 543.6

Evans Blue

20-abx082082
  • EUR 210.00
  • EUR 260.40
  • 1 g
  • 5 g

Evans Blue

20-abx082237
  • EUR 226.80
  • EUR 276.00
  • 1 g
  • 5 g

Evans Blue

HY-B1102 100mg
EUR 142.8

Dextran blue 2000

20-abx082076
  • EUR 260.40
  • EUR 427.20
  • 1 g
  • 5 g

Cell Navigator® Mitochondrion Staining Kit *Blue Fluorescence*

22665 500 Assays
EUR 222

Thymol Blue

GT9755-100G 100 g
EUR 180

Thymol Blue

GT9755-10G 10 g
EUR 60

Thymol Blue

GT9755-25G 25 g
EUR 84

Thymol Blue

GT9755-50G 50 g
EUR 112.8

Thymol Blue

GT9755-5G 5 g
EUR 50.4

Xylenol Blue

GT7988-1G 1 g
EUR 55.2

Xylenol Blue

GT7988-25G 25 g
EUR 180

Xylenol Blue

GT7988-5G 5 g
EUR 93.6

Meldola's blue

GT0259-10G 10 g
EUR 103.2

Meldola's blue

GT0259-25G 25 g
EUR 160.8

Meldola's blue

GT0259-50G 50 g
EUR 255.6

Calcein Blue

HY-101887 25mg
EUR 142.8

Diphenyl Blue

HY-D0970 10mM/1mL
EUR 135.6

Toluidine Blue

abx082425-5g 5 g
EUR 226.8

Thiazolyl Blue

HY-15924 10mM/1mL
EUR 135.6

Methylene Blue

HY-14536 100mg
EUR 142.8

Bromphenol Blue

20-abx082184
  • EUR 276.00
  • EUR 226.80
  • 25 g
  • 5 g

Bromphenol Blue

20-abx082376
  • EUR 292.80
  • EUR 226.80
  • 25 g
  • 5 g

Cell Navigator® F-Actin Labeling Kit *Blue Fluorescence*

22660 500 Tests
EUR 222

Disperse Blue 124

LCS-4848 EACH
EUR 388.8

Disperse Blue 148

HY-D0569 10mM/1mL
EUR 206.4

Bromophenol blue

abx183916-500ml 500 ml
EUR 276

Bromophenol blue

abx183916-5l 5l
EUR 710.4

Bromophenol Blue

CH019 25 g
EUR 158.4

Bromophenol Blue

CH020 50 g
EUR 199.2

Bromophenol Blue

CH021 100 g
EUR 272.4

Bromophenol blue

BB2230 25g
EUR 82.97

Bromophenol Blue

B7774-5000 5 g
EUR 104.4
Description: IC50: N/ABromophenol blue (3',3",5',5"-tetrabromophenolsulfonphthalein) is widely used in gel loading buffers. Bromophenol blue is experimentally used as acolor marker, apH indicator, and a dye.

Bromophenol Blue

abx082607-10g 10 g
EUR 243.6

Bromothymol Blue

GT6837-10G 10 g
EUR 56.4

Bromothymol Blue

GT6837-25G 25 g
EUR 73.2

Bromothymol Blue

GT6837-50G 50 g
EUR 103.2

Bromothymol Blue

GT6837-5G 5 g
EUR 50.4

Bromophenol Blue

GT2874-100G 100 g
EUR 151.2

Bromophenol Blue

GT2874-10G 10 g
EUR 62.4

Bromophenol Blue

GT2874-25G 25 g
EUR 79.2

Bromophenol Blue

GT2874-50G 50 g
EUR 103.2

Bromophenol Blue

GT2874-5G 5 g
EUR 54

Bromophenol Blue

G114 5.0 g
EUR 122.4

Bromothymol Blue

HY-D0012 1g
EUR 142.8

Bromoxylenol Blue

GT8506-10G 10 g
EUR 170.4

Bromoxylenol Blue

GT8506-1G 1 g
EUR 55.2

Bromoxylenol Blue

GT8506-5G 5 g
EUR 112.8

Phthalocyanine blue

20-abx181340
  • EUR 393.60
  • EUR 260.40
  • EUR 895.20
  • 100 g
  • 25 g
  • 500 g

Container 40ml PP Blue+Label+Blue Cap Select

CON1025 PK1000
EUR 254.4

Hydroxynaphthol blue

GT3182-100G 100 g
EUR 284.4

Hydroxynaphthol blue

GT3182-10G 10 g
EUR 93.6

Hydroxynaphthol blue

GT3182-25G 25 g
EUR 132

Hydroxynaphthol blue

GT3182-50G 50 g
EUR 199.2

Recombinant Trypanosoma Cruzi TOR Protein (aa 1-287) (Cell Free Expression)

VAng-Wyb8529-100g 100 µg
EUR 7797.6
Description: Trypanosoma Cruzi Tetraspanning orphan receptor protein, recombinant protein.

Recombinant Trypanosoma Cruzi TOR Protein (aa 1-287) (Cell Free Expression)

VAng-Wyb8529-10g 10 µg
EUR 3096
Description: Trypanosoma Cruzi Tetraspanning orphan receptor protein, recombinant protein.

Recombinant Trypanosoma Cruzi TOR Protein (aa 1-287) (Cell Free Expression)

VAng-Wyb8529-50g 50 µg
EUR 4744.8
Description: Trypanosoma Cruzi Tetraspanning orphan receptor protein, recombinant protein.

Bromochlorophenol Blue

GT2674-100MG 100 mg
EUR 93.6

Bromochlorophenol Blue

GT2674-1G 1 g
EUR 208.8

Recombinant Trypanosoma Brucei ATP6 Protein (aa 1-229) (Cell Free Expression)

VAng-Wyb8033-100g 100 µg
EUR 7582.8
Description: Trypanosoma Brucei brucei ATP synthase subunit a protein, recombinant protein.

Recombinant Trypanosoma Brucei ATP6 Protein (aa 1-229) (Cell Free Expression)

VAng-Wyb8033-10g 10 µg
EUR 2881.2
Description: Trypanosoma Brucei brucei ATP synthase subunit a protein, recombinant protein.

Recombinant Trypanosoma Brucei ATP6 Protein (aa 1-229) (Cell Free Expression)

VAng-Wyb8033-50g 50 µg
EUR 4530
Description: Trypanosoma Brucei brucei ATP synthase subunit a protein, recombinant protein.

Recombinant Trypanosoma Brucei SLS1 Protein (aa 1-355) (Cell Free Expression)

VAng-Wyb8217-100g 100 µg
EUR 8044.8
Description: Trypanosoma Brucei brucei Phosphatidylinositol:ceramide inositolphosphotransferase protein, recombinant protein.

Recombinant Trypanosoma Brucei SLS1 Protein (aa 1-355) (Cell Free Expression)

VAng-Wyb8217-10g 10 µg
EUR 3342
Description: Trypanosoma Brucei brucei Phosphatidylinositol:ceramide inositolphosphotransferase protein, recombinant protein.

Recombinant Trypanosoma Brucei SLS1 Protein (aa 1-355) (Cell Free Expression)

VAng-Wyb8217-50g 50 µg
EUR 4976.4
Description: Trypanosoma Brucei brucei Phosphatidylinositol:ceramide inositolphosphotransferase protein, recombinant protein.

Recombinant Trypanosoma Brucei SLS2 Protein (aa 1-323) (Cell Free Expression)

VAng-Wyb8223-100g 100 µg
EUR 7929.6
Description: Trypanosoma Brucei brucei Phosphatidylethanolamine:ceramide ethanolaminephosphotransferase protein, recombinant protein.

Recombinant Trypanosoma Brucei SLS2 Protein (aa 1-323) (Cell Free Expression)

VAng-Wyb8223-10g 10 µg
EUR 3228
Description: Trypanosoma Brucei brucei Phosphatidylethanolamine:ceramide ethanolaminephosphotransferase protein, recombinant protein.

Recombinant Trypanosoma Brucei SLS2 Protein (aa 1-323) (Cell Free Expression)

VAng-Wyb8223-50g 50 µg
EUR 4876.8
Description: Trypanosoma Brucei brucei Phosphatidylethanolamine:ceramide ethanolaminephosphotransferase protein, recombinant protein.

Recombinant Trypanosoma Brucei SLS3 Protein (aa 1-329) (Cell Free Expression)

VAng-Wyb8228-100g 100 µg
EUR 7946.4
Description: Trypanosoma Brucei brucei Phosphatidylcholine:ceramide cholinephosphotransferase 3 protein, recombinant protein.

Recombinant Trypanosoma Brucei SLS3 Protein (aa 1-329) (Cell Free Expression)

VAng-Wyb8228-10g 10 µg
EUR 3244.8
Description: Trypanosoma Brucei brucei Phosphatidylcholine:ceramide cholinephosphotransferase 3 protein, recombinant protein.

Recombinant Trypanosoma Brucei SLS3 Protein (aa 1-329) (Cell Free Expression)

VAng-Wyb8228-50g 50 µg
EUR 4893.6
Description: Trypanosoma Brucei brucei Phosphatidylcholine:ceramide cholinephosphotransferase 3 protein, recombinant protein.

Recombinant Trypanosoma Brucei SLS4 Protein (aa 1-365) (Cell Free Expression)

VAng-Wyb8233-100g 100 µg
EUR 8078.4
Description: Trypanosoma Brucei brucei Phosphatidylcholine:ceramide cholinephosphotransferase 4 protein, recombinant protein.

Recombinant Trypanosoma Brucei SLS4 Protein (aa 1-365) (Cell Free Expression)

VAng-Wyb8233-10g 10 µg
EUR 3360
Description: Trypanosoma Brucei brucei Phosphatidylcholine:ceramide cholinephosphotransferase 4 protein, recombinant protein.

Recombinant Trypanosoma Brucei SLS4 Protein (aa 1-365) (Cell Free Expression)

VAng-Wyb8233-50g 50 µg
EUR 5025.6
Description: Trypanosoma Brucei brucei Phosphatidylcholine:ceramide cholinephosphotransferase 4 protein, recombinant protein.

Recombinant Trypanosoma Cruzi COXII Protein (aa 1-210) (Cell Free Expression)

VAng-Wyb8407-100g 100 µg
EUR 7516.8
Description: Trypanosoma Cruzi Cytochrome c oxidase subunit 2 protein, recombinant protein.

Recombinant Trypanosoma Cruzi COXII Protein (aa 1-210) (Cell Free Expression)

VAng-Wyb8407-10g 10 µg
EUR 2815.2
Description: Trypanosoma Cruzi Cytochrome c oxidase subunit 2 protein, recombinant protein.

Recombinant Trypanosoma Cruzi COXII Protein (aa 1-210) (Cell Free Expression)

VAng-Wyb8407-50g 50 µg
EUR 4464
Description: Trypanosoma Cruzi Cytochrome c oxidase subunit 2 protein, recombinant protein.

Recombinant Trypanosoma Cruzi Tc00.1047053506885.124 Protein (aa 1-335) (Cell Free Expression)

VAng-Wyb8503-100g 100 µg
EUR 7962
Description: Trypanosoma Cruzi (strain CL Brener) Phosphatidylinositol:ceramide inositolphosphotransferase protein, recombinant protein.

Recombinant Trypanosoma Cruzi Tc00.1047053506885.124 Protein (aa 1-335) (Cell Free Expression)

VAng-Wyb8503-10g 10 µg
EUR 3261.6
Description: Trypanosoma Cruzi (strain CL Brener) Phosphatidylinositol:ceramide inositolphosphotransferase protein, recombinant protein.

Recombinant Trypanosoma Cruzi Tc00.1047053506885.124 Protein (aa 1-335) (Cell Free Expression)

VAng-Wyb8503-50g 50 µg
EUR 4910.4
Description: Trypanosoma Cruzi (strain CL Brener) Phosphatidylinositol:ceramide inositolphosphotransferase protein, recombinant protein.

Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Blue Fluorescence*

22634 100 Tests
EUR 334

Cell Explorer™ Live Cell Labeling Kit *Blue Fluorescence with 405 nm Excitation*

22614 200 Tests
EUR 222

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